PCNA与C-myc蛋白在卵巢上皮癌中的表达及临床意义

Clinical correlation of PCNA and C-myc protein expression in ovarian epithelial carcinoma

为探讨增殖细胞抗原（PCNA）及C－myc蛋白在卵巢上皮癌中的表达及临床意义，应用SABC免疫组织化学方法，对32例卵巢上皮癌、10例卵巢上皮瘤和6例正常卵巢组织的石蜡标本作PCNA和C－myc蛋白的检测，并分析卵巢上皮癌的临床病理资料。结果表明，正常卵巢组织、卵巢良性病变组织到卵巢上皮癌PCNA和C－myc蛋白的表达呈增高趋势；PCNA增殖指数与卵巢上皮癌临床分期、组织学分级高度负相关，与预后有关但不具统计学意义；C－myc蛋白表达与卵巢上皮癌的临床分期、预后无关，与组织学分级高度负相关。在卵巢上皮癌中，C－myc蛋白阳性表达与PCNA大量出现有极大相关性。说明C－myc蛋白检测可能不会成为早期诊断卵巢癌的方法。检测PCNA及C－myc蛋白可以估计卵巢上皮癌患者的预后，辅助指导临床化疗。

To investigate correlation of proliferating cell nuclear antigen(PCNA) and C-myc protein expression in ovarian epithelial carcinoma, two monoclonal antibodies, PCNA PC,, and antic-myc protein were used in ummunohistochemical reaction to measure PCNA and C-myc protein in paraffin section of 32 ovarian epithelial carcinoma, 10 ovarian epithelial tumor and 6 normal controls, and clinical pathological sechons of ovarian epithelial carcinoma were also examined. The results showed that there was no expression of PCNA and C-myc protein in normal controls,the genesis and development of ovarian epithelial carcinoma were correlated with a great deal of PCNA occurrence (P＜0. 01 ); PCNA proliferation exponent was correlated with the clinical stages and histodifferentiation of ovarian epithelial carcinoma (P ＜ 0.01 ),and also prognosis, but without statistical significance (P ＞0. 05 ); C-myc protein expression wasn' t correlated with the clinical stages and prognosis of ovarian epithelial carcinoma, but was correlated well with histodifferentiation (P＜ 0. 01 ); in ovarian epithelial carcinoma, C-myc protein expression was correlated well with a great deal of PCNA occurence (P ＜ 0.0l ). These suggested C-myc protein might not be a method in early ovarian carcinoma diagnosis.