亲和吸附离心管法检测甲胎蛋白异质体用于诊断肝癌的研究

The studies of alpha-fetoprotein isoforms using with centrifugal column method in diagnosis of hepatocelluar carcinoma

目的采用亲和吸附离心管法检测甲胎蛋白异质体(AFP-L3),分析甲胎蛋白异质体AFP-L3在诊断肝癌的临床应用价值。方法选择121例甲胎蛋白AFP阳性患者血清(≥10ng/ml)。使用亲和吸附离心管方法检测血清甲胎蛋白异质体AFP-L3,并且对研究对象进行跟踪,分析甲胎蛋白异质体用于临床在鉴别肝癌的作用。结果以甲胎蛋白异质体AFP-L3≥10作为诊断指标,肝癌患者血清中甲胎蛋白异质体AFP-L3异常升高者明显高于其他肝病患者(P<0.01);对58例临床确诊为肝癌的患者甲胎蛋白异质体AFP-L3的诊断灵敏度是79.31;在63例临床诊断为其他肝脏疾病的患者甲胎蛋白异质体AFP-L3的诊断特异性达到92.06,甲胎蛋白异质体AFP-L3的符合率85.95。结论应用亲和吸附离心管法检测甲胎蛋白异质体AFP-L3在肝癌与良性肝脏病变鉴别诊断中具有重要临床价值。

Objective To explore the clinical significance of alpha-fetoprotein-L3（AFP-L3） with centrifugal column method in the diagnosis of hepatocelluar carcinoma.Methods The serum samples of 121 patients with elevated AFP level（≥10ng/ml） were detected by chemiluminescence immunoassay for AFP and centrifugal column method for AFP-L3,and the clinical value of AFP-L3 were studied in the differentiation diagnosis of hepatocelluar cancer.Results The level of AFP-L3（%） in serum of patients with hepatocellular carcinoma was significantly higher than those in the patients with other liver diseases（P0.05）.Taking AFP-L3（%）≥10ng/ml as the diagnositic criteria,the sensitivity of AFP-L3 was 79.31% in 58 hepatocellular carcinoma patients and the specificity of AFP-L3 was 92.06% in 63 other liver diseases patients.The diagnostic accuracy was 85.95%.Conclusion Detection of AFP-L3 seemed be of clinical value in diagnosis and differential diagnosis of hepatocellular carcinoma.