高效液相色谱法测定奶粉中的β-胡萝卜素

Determination of β-Carotene in Milk Powder by HPLC

采用高效液相色谱法测定奶粉中的β－胡萝卜素。奶粉样品在甲醇溶剂中碱化后，用石油醚萃取β－胡萝卜素，经Ｖａｒｉａｎ３０ｃｍ×４ｍｍ硅胶柱分离，紫外检测定量，该方法最低检出限为５．３８×１０－１０ｇ，回收率为９５．３％～９８．４％，变异系数ＣＶ（％）为２．０４％

This method is for determination of β-carotene in milk powder by HPLC. The sample was treated with a basic methanol solution,and then β-carotene in sample was extracted by using petroleum ether,seperated in Varian 30cm×4mm Si-5 columm and detected at UV453 nm. The minimum detectable value is 5.38×10 -10 g, the recovery is 95.3%～98.4%,the variation coefficient CV(%) is 2.04%.