亚慢性氟染毒对大鼠骨组织病理改变和β-连环蛋白表达的影响

Effect of subchronic fluoride exposure on pathologic change and β-连环蛋白 expression in rat bone tissue

目的观察亚慢性氟染毒对大鼠骨组织中β-连环蛋白表达的影响,探讨β-连环蛋白在氟致骨损伤中的可能作用。方法 40只Wistar大鼠按体重随机分成4组,染氟组大鼠饮用含氟50、100和150mg/L的自来水,对照组大鼠饮用自来水,3个月后测定大鼠血清及骨组织氟含量,Real-time RT-PCR法和免疫组化法检测骨组织中β-连环蛋白mRNA和蛋白质表达情况。结果 HE染色显示染氟组大鼠病理学改变呈现骨皮质增厚、骨小梁增多等骨硬化表现,骺板肥大软骨细胞大量堆积且排列紊乱。染氟组大鼠骨组织中β-连环蛋白mRNA表达显著高于对照组,差异有统计学意义(P<0.01),而且随染毒剂量升高,β-连环蛋白mRNA表达逐渐升高。免疫组化显示染氟组大鼠骨组织中β-连环蛋白主要由成骨细胞和破骨细胞表达,骺板软骨肥大细胞也有阳性表达。结论β-连环蛋白在亚慢性氟染毒大鼠骨质硬化的发生中发挥重要作用。

Objective To explore the effect of subchronic fluoride exposure on the expression of β-catenin in the bone of rats and the role of β-catenin in skeletal damage.Methods Wistar rats were randomly divided into four groups.The rats were treated at the doses of 50,100 and 150mg/L F-in drinking water,and control rats were drunk tap water.The contents of fluoride in bone and serum were determined after three month.The expressions of β-catenin were analyzed by real-time RT-PCR and immunohistochemistry.Results HE staining indicated that the compact bone was thicker and the trabecular bone increased in fluoride-treated group in comprison with control group.The hypertrophic chondrocytes accumulated and arranged disordered in fluoride-treated rats.The expressions of β-catenin mRNA and protein expressions in bones of fluoride-treated rats were higher than those of control group.Immunohistochemistry indicated that β-catenins were mainly expressed by osteoblast and osteoclast.There was also positive staining in hypertrophic chondrocyte.Conclusion It was suggested that β-catenin could play important role in bone sclerosis of subchronic fluoride exposure.