红细胞膜带3蛋白水平与中老年人生理状况相关分析

Correlation of band 3 protein in erythrocytes with physiological status of middle-aged and elderly people

目的通过对红细胞膜带3蛋白水平与中老年人生理状况相关因素关系的分析,了解带3蛋白在不同生理状态下表达的差异。方法采取整群随机抽样的方法收集中老年人体检资料及血样218例,其中男性80例,女性138例,年龄45～100岁。制备红细胞膜,采用SDS-聚丙烯酰胺凝胶电泳技术分析红细胞膜带3蛋白水平。不同分组间带3蛋白水平的比较采用独立样本t检验或方差分析,相关回归分析采用Pearson相关和线性回归。结果红细胞膜带3蛋白水平与体力活动水平(r=0.149)呈正相关(P<0.05),与收缩压(r=-0.156)呈负相关(P<0.05)。带3蛋白水平与性别、年龄、BMI、舒张压无相关关系(P>0.05)。中等体力活动者带3蛋白含量为24.09%,明显高于轻体力活动者23.42%(P<0.05)。收缩压升高者带3蛋白含量为23.33%,明显低于正常人水平24.20%(P<0.05)。结论伴随着体力活动的增加和收缩压的降低带3蛋白水平升高。

Objective To analyze the correlation of band 3 protein of erythrocytes with physiological conditions of middle-aged and elderly people,and to investigate the expression of band 3 protein under different physiological status.Methods Cluster randomized sampling method for selecting subjects.Physical examination data and blood samples of 218 middle-aged and elderly people（80 males and 138 females） were collected.SDS-polyacrylamide gel electrophoresis was used to analyze band 3 protein in erythrocyte membrane.The difference of band 3 protein in different groups was analyzed by T Test and One-Way ANOVA.Correlation and regression of band 3 protein with physiological status was analyzed by Pearson correlation and linear regression.Results There was a significantly positive correlation（r=0.149）between the level of band 3 protein and physical activity（P0.05）,and a negative correlation（r=-0.156）between the level of band 3 protein and systolic blood pressure（P0.05）.The correlation of band 3 protein with gender,age,BMI and diastolic blood pressure（P0.05） is not significant.The band 3 protein level of workers with medium activity was 24.09%,which was significantly higher than that of light activity workers（23.42%）（P0.05）.The subjects with hypertension were proved to have a significantly lower level of band 3 protein（23.33%） than normal individuals（24.20%）（P0.05）.Conclusion The level of band 3 protein was positively correlated with physical activity and negatively correlated with systolic blood pressure.