^(60)Coγ射线对生理盐水和玉米中黄曲霉孢子的辐照效应研究

Irradiation effect of ^(60)Co gamma ray on Aspergillus flavus spores in normal saline and maize

目的研究γ射线对黄曲霉孢子辐照效应。方法两种介质中不同浓度黄曲霉孢子经不同剂量辐照后进行菌落计数,计算D10值和理论最小杀灭剂量,并实验验证实际最小杀灭剂量。结果生理盐水中高浓度孢子D10值0.39kGy,最低杀灭剂量2.5kGy,低浓度孢子D10值0.47kGy,最低杀灭剂量1.5kGy;玉米中高浓度孢子D10值为0.67kGy,最低杀灭剂量3.0kGy,低浓度孢子D10值为0.72kGy,最低杀灭剂量2.0kGy。结论 3.0kGy可用于粮食中黄曲霉的杀灭。

Objective To observe irradiation effect of gamma ray on Aspergillus flavus spores.Methods Aspergillus flavus spores of different concentration were prepared on two media and were exposed to gamma ray.D10 values and theoretical minimal sterilizing doses were calculated by colony counting results.Actual minimal sterilizing doses were verified according to theoretical dose.Results D10 value and minimal sterilizing dose of high concentration spore in normal saline were 0.39kGy and 2.5kGy.Those of low concentration spore in normal saline were 0.47kGy and 1.5kGy.D10 value and minimal sterilizing dose of high concentration spore in maize were 0.67kGy and 3.0kGy.Those of low concentration spore in maize were 0.72kGy and 2.0kGy.Conclusion 3.0kGy may be suitable for grain processing to sterilize Aspergillus flavus.