基质辅助激光解析电离飞行时间质谱快速鉴定耐甲氧西林金黄色葡萄球菌

Rapid method study of methicillin-resistant Staphylococcus aureus identified by Matrix-Assisted laser desorption Ionization-Time of flight mass spectrometry

目的研究基质辅助激光解析电离飞行时间质谱(Matrix-Assisted Laser Desorption Ionization-Timeof Flight Mass Spectrometry,MALDI-TOF-MS)用于快速检测鉴定金黄色葡萄球菌及其耐药性的可行性。方法用MALDI-TOF-MS和API Staph系统鉴定了40株临床分离的葡萄球菌,用Kirby-Bauer法检测金黄色葡萄球菌的耐药性。结果 40株临床分离的葡萄球菌中34株细菌用MALDI-TOF-MS鉴定为金黄色葡萄球菌,6株为其他葡萄球菌,与API Staph系统鉴定结果一致。Kirby-Bauer法耐药性检测显示其中11株为耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA),23株为甲氧西林敏感金黄色葡萄球菌(methicillin-susceptible Staphylococcus aureus,MSSA),34株金黄色葡萄球菌用MALDI-TOF-MS鉴定时聚类为2类,结果与耐药性检测结果一致。结论结果提示MALDI-TOF-MS可以用于MRSA的快速鉴定。

Objective To establish the method on rapid identification of Staphylococcus aureus and the performance of methicillin-resistance.Methods 40 isolation strains of staphylococcal were analyzed by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry（MALDI-TOF-MS） and API Staph Ident system,and the performance of methicillin-resistance was tested by Disk Diffusion Susceptibility Testing.Results 34 isolation strains were identified by MALDI-TOF-MS as Staphylococcus aureus,and the result was uniform to the API Staph Ident system.11 isolation strains were identified as methicillin-resistant Staphylococcus aureus（MRSA） and 23 isolation strains were methicillin-susceptible to Staphylococcus aureus（MSSA）.All the 34 isolation strains were clustered to 2 species identified by MALDI-TOF-MS.The result was uniform to the Disk Diffusion Susceptibility Testing.Conclusion These results demonstrated that MALDI-TOF-MS could be a rapid technique for identification and discrimination of MRSA.