脑康Ⅱ号干预糖尿病认知障碍大鼠血清3-脱氧葡糖醛酮的研究

Intervention of Serum 3-deoxyglucosone by Naokang Ⅱ in Rats with Diabetes Mellitus and Cognitive Impairment

目的:研究脑康Ⅱ号对糖尿病大鼠学习记忆功能的干预情况。方法:将3月龄雄性Wistar大鼠分为对照组、模型组、脑康Ⅱ号干预组(分为高、中、低剂量3组),模型组和脑康Ⅱ号干预组用链脲佐菌素60 mg·kg-1剂量ip,对照组ip相同体积的枸橼酸缓冲液,脑康Ⅱ号干预组分别使用20,10,5 g·kg-1的脑康Ⅱ号ig,采用水迷宫实验和气相色谱-质谱联用仪,分别观察糖尿病大鼠的行为学改变和血清3-脱氧葡糖醛酮(3-DG)的变化。结果:造模后模型组和中药干预组大鼠血糖较对照组显著升高(P<0.01)。Morris水迷宫检测大鼠到达站台时间显示,模型组需(18.6±6.3)s时间较对照组(7.5±3.1)s明显延长,具有显著性差异(P<0.01)。脑康Ⅱ号各治疗组所需时间(低、中、高剂量组分别为(9.9±3.2)s,(8.7±4.1)s,(10.1±3.9)s,较模型组明显减少(P<0.01)。糖尿病大鼠模型血清中3-DG含量(20.19±2.62)ng.mL-1,较对照组显著升高(P<0.05),约为对照组(7.02±1.19)ng.mL-1的2.5倍。脑康Ⅱ号高剂量治疗组3-DG含量较模型组显著降低(8.89±1.09)ng.mL-1(P<0.05)。结论:脑康Ⅱ号能够通过降低大鼠血清3-脱氧葡糖醛酮的水平,改善糖尿病大鼠认知障碍。

Objective：To study the influence of Naokang Ⅱ on diabetes mellitus and cognitive impairment in rats.Method：We used Naokang Ⅱ to treat rats with diabetes mellitus and cognitive impairment.We observed the change of rat＇s ability of learning and memory function,by the application of water maze,and examined the blood serum 3-deoxyglucosone using gas chromatography-mass spectrometry.Result：Naokang Ⅱcould not reduce rats serum glucose,but it could significantly reduce the swimming time to platform and serum 3-deoxyglucosone.Conclusion：Naokang Ⅱ could improve diabetes mellitus rat＇s cognitive impairment,by reducing serum 3-deoxyglucosone.