小柴胡汤君臣药及其配伍提取物对感染CVB_(3m)心肌细胞的血清药理学研究

Serum Pharmacology of Xiaochaihutang Jun and Chen Herbs and Their Compatibility Extract on Myocardial Cells Infected CVB_(3m)

目的:探讨小柴胡汤(XCT)君臣药及其配伍提取物对感染CVB3m心肌细胞的抗病毒与保护作用,并试阐述机制。方法:制取3种提取物的含药血清,并测定体外对CVB3m毒力的影响。培养乳鼠心肌细胞建立心肌炎模型,从3个方面检测:应用MTT法检测含药血清对心肌细胞的保护率;检测各孔上清液中乳酸脱氢酶(LDH)及天冬氨酸氨基转移酶(AST)的释放量;RT-PCR法检测感染CVB3m的心肌细胞中CVB3m的含量。结果:黄芩提取物含药血清对CVB3m有直接灭活的作用。3个方面检测结果均显示:含药血清组与空白血清组比较,有显著性差异(P<0.01),黄芩组和配伍组分别与柴胡组比较,均有显著性差异(P<0.01),配伍组与黄芩组比较,无显著性差异。结论:黄芩提取物有直接灭活病毒的作用,黄芩和配伍提取物具有明显的抑制病毒复制和对病毒感染后细胞的保护作用,等量配伍提取物没有表现出作用增强的优势。

Objective：To explore Xiaochai hutang（XCT＇s） jun and chen herbs and their compatibility extracts antivirus and the protective function by infected CVB3m and to elaborate the therapeutic mechanism.Method：We prepared herb-contained serum of the extracts from Bupleurum,Scutellaria and their compatibility,and measured effects on virulence CVB3m in vitro.We cultured the myocardial cells of suckling mice and established the viral myocarditis mAel.From three aspects tested： the protection rate of the myocardial cell after infected CVB3m by MTT methA;the release of LDH and AST in the supernatant of each hole;and CVB3m content of containing serum on myocardial cells infected CVB3m by RT-PCR.Result：Scutellaria herb-contained serum might have affect on directly kill CVB3m in vitro.Three results were： a significant difference though containing serum group compared with the blank serum（P0.01）;Scutellaria group and the compatibility group were compared with the Bupleurum group,there were significant differences（P0.01）;Compatibility group compared with the Skullcap group was no significant difference.Conclusion： Scutellaria extract might have affect on directly kill CVB3m in vitro.Scutellaria and the compatibility extracts inhibited the virus replication and protected cells infected virus.Equivalent compatibility extract did not show the advantage of the enhanced role.