卷柏提取物对去势大鼠股骨中羟脯氨酸含量的影响

Experimental Study of Extract of Selaginella tamariscina on Content of Hydroxyproline in Femur of Ovariectomized Rats

目的:采用柱前衍生化法对去势大鼠股骨中羟脯氨酸(HyP)的含量进行测定,观察卷柏提取物对去势大鼠股骨中HyP含量的影响。方法:Wistar雌性大鼠摘除卵巢后1周开始ip卷柏总提物665 mg·kg-1,卷柏正丁醇部位85 mg·kg-1,卷柏水部位490 mg·kg-1,持续11周后处死,取其股骨,采用2,4-二硝基氯苯柱前衍生化反相高效液相色谱法,观察卷柏提取物对去势大鼠股骨中HyP含量的影响。结果:HyP 0.125～2.00 g.L-1线性关系良好(r=0.999 4),精密度试验RSD<1%,24 h内稳定性试验RSD<5%,加样回收率均在90%～110%;卷柏水部位和正丁醇部位均能增加去势大鼠股骨中HyP含量。结论:卷柏提取物能增加去势大鼠股骨中HyP含量,其中卷柏水部位和正丁醇部位,对去势大鼠骨质疏松具有一定的干预作用。

Objective：To study the effect of the extract of Selaginella tamariscina on the content of hydroxyproline in femur of ovariectomized rats.Method：Wistar rats were ovariectomized except for the normal group.One week after operation,the rats were treated with different medicines.Eleven weeks after treated the medicines,the rats were killed,the content of hydroxyproline in femur of ovariectomized rats was determined by RP-HPLC with pre-column derivatization by 2,4-dinitrochlorobenzene（DNCB）.Result：Calibration curves showed good linearity within the concentration range of 0.125-2.00 g·L-1 for hydroxyproline.The RSD of precision was less than 1%,the RSD of stability of the sample was less than 5% in 24 hours and recoveries of assay were 90%-110%.The water part group and n-butanol part group of S,tamariscina could increase the content of hydroxyproline in femur of ovariectomized rats.Conclusion：The extract of S.tamariscina can increase the content of hydroxyproline in femur of ovariectomized rats,suggesting that the water part group and n-butanol part group of S.tamariscina can intervene the osteoporosis in ovariectomized rats.