大鼠真菌性腹腔炎症渗出液的上清液抗真菌作用研究

Fungicidal Effect of Peritoneal Exudates Supernatant in Rats Infected by Candida albicans

目的:观察大鼠真菌性腹腔炎症渗出液的上清液中的抗菌物质对真菌的杀菌作用。方法:给大鼠ip活的白色念珠菌Candida albicans造成真菌感染模型,观察腹腔炎症渗出液的上清液对C.albicans、光滑念珠菌C.glabrata、热带念珠菌C.tropicalis的杀菌作用,观察温度对上清液抗真菌作用的影响,并在显微镜下观察杀灭C.albicans的过程。结果:造模后24 h的腹腔炎症渗出大量渗出液,渗出液上清液中含有大量的蛋白质,渗出液上清液对C.albicans,C.glabrata,C.tropicalis具有强的杀菌作用,杀菌作用受温度的影响,56℃处理后杀菌活性几乎丧失,上清液可使C.albicans菌体萎缩,然后胞体破碎死亡。结论:大鼠真菌性腹腔炎症渗出液上清液中存在强的抗真菌物质,具有强的抗真菌作用,可能通过对真菌的细胞壁/细胞膜而发挥作用。其可能是未被关注或未发现的抗菌蛋白/肽。

Objective：To investigate the fungicidal activity of the substance in peritoneal exudates from inflammatory fluid of abdominal cavity in rats infected by Candida albicans.Method：The model of severe abdominal cavity inflammation was prepared by intraperitoneal administration of C.albicans to rats.The peritoneal exudates were collected and the fungicidal activity of peritoneal exudation supernatant were determined.Result：The peritoneal exudation supernatant collected at 24 h possessed the most potent fungicidal activity.The fungicidal activity of 80% peritoneal exudation supernatant collected at 24 h was dramatically declined after incubated in 56 ℃for 10 min.C.albicans morphology changed gradually and their color turned dark,their thalline was shrinked and their cell wall was degraded after treated by peritoneal exudation supernatant for 30 min,C.albicans color turned dark further,the thalline was smaller further,and then was expired due to disruption lastly.Conclusion：The supernatant of imflammatory peritoneal exudates from abdominal cavity inflammation rat models infected by C.albicans possess potent fungicidal activity against fungus,the substances may be unknown fungicidal protein/ peptide.