乙肝转阴颗粒对D-半乳糖胺诱导小鼠急性肝损伤的保护作用

Protection of Yigan Zhuanyin Granules on Acute Liver Injuries Induced by D-galactosamine in Mice

目的:研究乙肝转阴颗粒(YGZYDG)对D-半乳糖胺(D-GalN)所致小鼠急性肝损伤的保护作用,并探讨其作用机制。方法:观测YGZYDG(1.68,0.84,0.42 g·kg-1)连续ig 7 d,对ip D-GalN(0.8 g·kg-1)致急性肝损伤模型小鼠血清门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),碱性磷酸酶(AKP)活性,血清白蛋白(Alb)含量,总抗氧化能力(T-AOC)以及肝组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性的影响。结果:与模型组比较,YGZYDG能降低血清AST,ALT和AKP的活性及肝组织MDA含量;提高血清Alb含量,T-AOC能力和肝组织SOD活性(P<0.01或P<0.05);能够减轻肝细胞损伤程度。结论:YGZYDG对D-半乳糖胺所致的急性肝损伤具有保护作用,其机制可能与其抗氧自由基、抑制脂质过氧化作用有关。

Objective：To study the effect of Yigan Zhuanyin granules（ZGZYG）on acute liver injuries induced by D-galactosamine（D-GalN）in mice and its mechanism.Method：After oral administration of YGZYG（1.68,0.84,0.42 g·kg-1）for seven days,the mice were abdominally administrated D-GalN（0.8 g·kg-1）to induce liver injury.The activities of AST,ALT and ALP,the content of Alb,the ability of T-AOC in serum,the content of MDA,and the activity of SOD in liver tissue were investigated.Result：Compared with model group,the activities of AST,ALT and AKP,and the content of MDA in the treatment groups were decreased;while the content of Alb,the ability of T-AOC,and the activity of SOD were increased（P0.01 or P0.05）.The degree of hepatic injury was lessened.Conclusion：YGZYG has protective effect on acute liver injury induced chemically.The mechanism may be related to attenuating free radical and inhibiting the effect on lipid peroxidation.