红果槲寄生抗肿瘤有效部位的确定

Determination of Effective Part of Anti-tumor Activity in Viscum coloratum (Kom.)Nakai f.Rubroaurantiacum Kitag

目的:确定红果槲寄生[Viscum coloratum(Kom.)Nakai f.Rubroaurantiacum Kitag,VCR]的抗肿瘤有效部位。方法:以S180实体瘤小鼠作为动物模型,以抑瘤率为考察指标,对红果槲寄生75%乙醇提取物的不同溶剂萃取部位:石油醚萃取部分、氯仿萃取部分、乙酸乙酯萃取部分、正丁醇萃取部分以及水层部分分别进行了抗肿瘤活性筛选。将接种肿瘤的昆明小鼠在成瘤后按照瘤体大小随机分为7组,阳性对照组于ip环磷酰胺,给药剂量为100 mg·kg-1,其他试验组于同一时间ig给药,每天1次,给药剂量按生药量计为14.2 g·kg-1,空白对照组给予相同剂量的空白溶剂,连续给药8 d。结果:红果槲寄生提取物能明显抑制小鼠S180实体瘤生长,其中正丁醇萃取物组,平均抑瘤率为48.8%;氯仿萃取物组,平均抑瘤率为44.1%。结论:正丁醇和氯仿萃取部分抗肿瘤活性较强,为红果槲寄生抗肿瘤有效部位。

Objective：ascertain the effective part of anti-tumor activity in Viscum coloratum（Kom.）Nakai f.Rubroaurantiacum Kitag（VCR）.Method：We made sarcoma S180 mice as the animal model,the inhibition rate of tumor was used as investigation index.We carried out the screen assay of the anti-tumor for different solvent extraction part of the VCR extracts by 75% alcohol,including petroleum extraction part,chloroform extraction part,acetate extraction part,n-butanol extraction part and the rest.We randomly divided mice with inoculated tumor into 7 groups according to tumor size.The positive control group of mice were given ip cyclophosphamide of 100 mg·kg-1,the other experimental group at the same time were given ig of 14.2 g·kg-1·d-1 and the blank control group were given the same volume of the blank solvent,continuously administrated for 8 days.Result：The results showed that the extraction of VCR could obviously prevent the growing of the tumor in mice.The inhibition rate of tumor for the chloroform extraction part was 48.8%;the rate of the n-butanol extraction part was 44.1%.Conclusion：The chloroform extraction part and the n-butanol extraction part are the effective part of anti-tumor from VCR.