益气化瘀方对产后子宫复旧不全模型大鼠TNF-α,IL-1,6-K-PGF_(1α),TXB_2的影响

Effect of Yiqi Huayu Fang on Tumor Necrosis Factor-α, Interleukin-1,6-Keto-prostaglandin F_(1α),Thromboxane B_2 in Rat Model of Subinvolution of Uterus

目的:探讨益气化瘀方对产后子宫复旧不全模型大鼠肿瘤坏死因子α(TNF-α)、白介素-1(IL-1)、6-酮-前列腺素F1α(6-K-PGF1α)、血栓素B2(TXB2)的影响。方法:将72只雌鼠随机分为空白组、模型组、产复康组及益气化瘀方低、中、高剂量组(4.68,9.36,18.72 g·kg-1)。除空白组外,其余各组均造成子宫复旧不全模型,并于造模后第1～7天分别ig给药。采用双抗体夹心酶联免疫吸附法(ELISA)测定TNF-α值;采用免疫组化法检测IL-1含量;采用放免药盒测试法测定6-K-PGF1α和TXB2的含量。结果:与空白组相比,模型组IL-1平均灰度、TXB2显著降低(P<0.01),其余各指标升高(P<0.05,P<0.01)。与模型组相比,产妇康组6-K-PGF1降低无统计学意义,其余各指标变化差异性显著(P<0.05,P<0.01)。益气化瘀方中剂量组IL-1平均灰度值升高、IL-1面密度值及TNF-α值降低有统计学意义(P<0.05,P<0.01);益气化瘀方高剂量组各指标变化均有统计学意义(P<0.05,P<0.01)。与产妇康组相比,益气化瘀方高剂量组降低6-K-PGF1水平的作用较显著。而益气化瘀方各剂量组间比较,益气化瘀方高剂量组能更好调节PGI2/TXA2比值。结论:益气化瘀方能降低炎症因子TNF-α,IL-1水平,有抗炎作用;能调节PGI2/TXA2比值,促进PGI2/TXA2之间的动态平衡,使血止而不留瘀,从而发挥治疗产后子宫复旧不全之功效。益气化瘀方高剂量组疗效显著。

Objective：To explore the effect of Yiqi Huayu Fang on tumor necrosis factor-α（TNF-α）,interleukin-1（IL-1）,6-keto-prostaglandin F1α（6-K-PGF1α）,thromboxane B2（TXB2）in rats model of subinvolution of uterus.Method：Seventy-two SD female rats were randomly divided into 6 groups：blank group,model group,Chanfukang group and small-,middle-,large-dose of Yiqi Huayu Fang group.The model of subinvolution of uterus was established for rats in the groups beside the blank one.All rats in each group were administered by intragastric infusion with corresponding drugs during day 1-7 after the model established.The contents of TNF-α,IL-1,6-K-PGF1α and TXB2 were respectively detected by the method of enzyme-linked immunosorbnent assay（ELISA）,immunohistochemistry and radioimmunoassay（RIA）.Result：Compared with blank group,the content of average gray level of IL-1 and TXB2 in model group decreased significantly（P0.01） while other indexes in this group became higher（P0.05,P0.01）.Compared with model group,other indexes in Chanfukang group changed significantly（P0.05,P0.01）except 6-K-PGF1;there had not obvious effects in small-dose of Yiqi Huayu Fang group;average gray level of IL-1 increased,while area density and TNF-α decreased in middle-dose of Yiqi Huayu Fang group,these changes were significant（P0.05,P0.01）;All indexes in large-dose of Yiqi Huayu Fang group changed apparently.Compared with Chanfukang group,large-dose of Yiqi Huayu Fang group had a more remarkable effect on reducing 6-K-PGF1 level.Group comparison among small-,middle-,large-dose of Yiqi Huayu Fang,large-dose group could regulating PGI2/ TXA2 ratio better.Conclusion：Yiqi Huayu Fang has anti-inflammatory effect by reducing the content of TNF-α and IL-1;It can also adjust PGI2/TXA2 ratio to maintain dynamic equilibrium and to got hemostasis without blood stasis.Through these ways,Yiqi Huayu Fang plays a role in the treatment of subinvolution of uterus.Large-dose of Yiqi Huayu Fang had a significant curative effect.