芽孢杆菌Z5溶铜绿微囊藻特性研究

Characteristics study of lysis of Microcystis aeruginosa by Bacillus. Z5.

从利用铜绿微囊藻液人工驯化的活性污泥中分离出一株编号为Z5的溶藻细菌,通过生理生化实验及16S rDNA序列分析,鉴定为芽孢杆菌.研究了Z5菌对铜绿微囊藻(Microcystis aeruginosa PCC 7806)的溶藻能力,考察了不同生长期的Z5菌无菌滤液及添加比例对铜绿微囊藻生长的影响.结果表明,作用6d后,Z5菌稳定期的无菌滤液对处于稳定期的铜绿微囊藻的去除效果达到91.36%.在4%～12%的投加范围内,无菌滤液的投加量与对铜绿微囊藻生长的抑制作用和溶解作用呈正比.研究表明芽孢杆菌Z5是通过分泌具有部分热稳定性的溶藻活性物质进行溶藻.

One algae-lysing bacterium strain named Z5 was isolated from an active sludge microorganism system that was artificially domesticated using Microcystis aeruginosa（PCC 7806）.The bacterium strain was identified as Bacillus.by the physiological and biochemical experiment and the sequence analysis of 16S rDNA.The capability of bacterial lysis of Microcystis aeruginosa by Bacillus.Z5 was studied and the influences of the bacteria-free filtrates of Bacillus.Z5 in different growth periods and the different adding volumes of the bacteria-free filtrates of Bacillus.Z5 on the growth of Microcystis aeruginosa were investigated as well.91.36% of Microcystis aeruginosa in stationary growth period had been removed in 6 days after addition of the bacteria-free filtrate of the stationary phase bacterium（Bacillus.Z5）.In certain range of addition volumes,from 4% to 12%,a positive correlation was found between algicidal activity and addition volumes of the bacteria-free filtrates of Bacillus.Z5.Microcystis aeruginosa was lysed by Bacillus.Z5 through most of the active algicidal substances which have thermal stability.