微柱离心-紫外分光光度法测定超氧化物歧化酶模拟物脂质体的包封率

Determination of entrapment efficiency of superoxide dismutase mimic liposomes by mini-column centrifugation-UV spectrophotometry

目的:建立超氧化物歧化酶(superoxide dismutase,SOD)模拟物脂质体包封率的测定方法。方法:利用紫外分光光度法测定SOD模拟物的含量,检测波长为371 nm。以薄膜分散法制备SOD模拟物脂质体,采用葡聚糖凝胶-微柱离心-紫外分光光度法测定其包封率。结果:SOD模拟物在60.0～240.0μg.mL-1范围内线性关系良好(r=0.999 3),微柱离心法能有效地将脂质体与游离药物分离,加样回收率测定结果为(99.18±1.05)%,利用该方法测定的SOD模拟物脂质体包封率为(91±1.63)%。结论:紫外分光光度法可以作为SOD模拟物的含量测定方法,微柱离心法操作简单,重现性好,适用于SOD模拟物脂质体包封率的测定。

Objective： To establish a method for determination of entrapment efficiency of superoxide dismutase（SOD） mimic liposomes.Methods： The concentration of SOD mimics was determined by UV spectrophotometry,and the UV detective wavelength was set at 371 nm.SOD mimic liposomes were prepared by the film-dispersion method,and the entrapment efficiency of the liposomes was determined by Sephadex G-50 mini-column centrifugation-UV spectrophotometry.Results： A calibrated linear curve of SOD mimic concentrations was within 60.0～240.0μg·mL-1（r=0.9993）.The liposomes and the free SOD mimics were well separated by mini-column centrifugation method.The recovery rate of SOD mimics was（99.18±1.05）% as compared with blank liposomes,and the entrapment efficiency of SOD mimic liposomes was（91±1.63）%.Conclusion： The UV spectrophotometry can be used for determination of the concentrations of SOD mimics.The mini-column centrifugation method is simple and reproducible,and applicable for determination of entrapment efficiency of SOD mimic liposomes.