拟除虫菊酯类农药人工抗原的合成与鉴定

Synthesis and identification of artificial antigens for pyrethroids

目的合成拟除虫菊酯类农药人工抗原并对其进行鉴定。方法采用功夫菊酸和3-间苯氧甲酸2种拟除虫菊酯类农药中间体,与四碳"间隔臂"——γ-氨基丁酸反应合成2种半抗原,经质谱鉴定合成成功。以牛血清蛋白为载体,采用活化酯法制备拟除虫菊酯的2种免疫原;以卵清蛋白为载体,采用混合酸酐法制备了3种包被原;经紫外光谱鉴定,粗略计算了偶联物最终浓度及结合比。结果制备的免疫原免疫Balb/c小鼠后得到的小鼠多抗血清经ELISA检测效价达1∶50 000以上,IC50达200 ng/ml。结论拟除虫菊酯人工抗原的制备成功。

Objective To synthesize and identify artificial antigens for pyrethroids.MethodsTwo haptens were synthesized by using Kung Fu chrysanthemic acid and 3-phenoxybenzoicacid as the intermediate of pyrethroids to react with a four carbon ＂spacer arm＂——aminobutyric acid,which were verified by MS.Immunogens for immunizing Balb/c mouse were prepared by the N-hydroxysuccinimide-activated ester method using bovine serum albumin as carriers;and coating antigens were prepared by mixed anhydride reaction using ovalbumin as carriers.The concentration of conjugates and molar ratios were estimated by UV spectrophotometry.Results The titer of polyclonal antisera determined by ELISA was high（〉1∶50000） and the IC50 was 200 ng/ml.ConclusionThe artificial antigens for pyrethroids were prepared successfully.