硫酸氨基葡萄糖亚急性毒性研究

Subacute toxicity of glucosamine sulfate

目的了解硫酸氨基葡萄糖的毒性。方法 80只SD大鼠分成4组,雌雄各半,试验剂量设为0.75、1.5、3.0 g/kg BW及空白对照组,将受试物均匀拌入饲料中喂养30 d,每周称重,记录饲料消耗量,试验结束时摘眼球采血作血常规及生化指标测定,然后取肝、肾、脾、胃称重,同时作组织病理学检查。结果受试物各剂量组大鼠体重增重、食物利用率与对照组比较,差异均无统计学意义;血红蛋白含量、红细胞计数、白细胞计数及白细胞分类均在正常值范围内;血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、总胆固醇、甘油三酯、血糖、总蛋白、白蛋白测定值均在正常值范围内;各剂量组大鼠的肾体比、脾体比、肝体比、睾体比与对照组比较,差异均无统计学意义;各剂量组大鼠的肝、肾、胃、脾切片染色后光镜检查结果未见特异性病理改变。结论硫酸氨基葡萄糖喂养30 d对动物机体未产生毒性,提示作为保健食品人们长期服用是安全无毒副作用的。

Objective To study whether the long term use of glucosamine sulfate is harmful to people.MethodsEighty SD rats were divided randomly into 4 groups by body weight,with 10 male rats and 10 female rats in each group.Then rats were fed with glucosamine sulfate at the dosage of 0,0.75,1.5 and 3.0 g/kg for 30 days,and were weighted once every week.Blood samples from eyeballs were collected for blood routine test and biochemical indexes.Liver,kidney,spleen and stomach of rats were weighted and used for histopathological examination.ResultsThe weight gain of rats in all treated groups was similar to that in non-treated group.Hb,RBC,WBC and leucocyte differential count of rats in all treated groups were normal.AST,ALT,BUN,Cr,TC,TG,Glu,Alb and TP of rats in all treated groups were normal.Kidney/body,spleen/body,liver/body and testicle/body coefficients of rats in all treated groups were similar to those in non-treated group.No pathological changes in liver,kidney,stomach and spleen of rats in all treated groups were observed under microscope.ConclusionNo adverse effect was observed in rats fed with glucosamine sulfate for 30 days,which indicate that long term use of glucosamine sulfate might be safe for people.