响应面法优化红树林内生真菌#3895产甘露醇的培养基

Optimization of culture medium of mannitol-producing from Mangrove endophytic fungus #3895 by response surface methodology

采用Plackett-Burman设计和响应面设计相结合的方法优化甘露醇发酵培养基组成,利用SAS软件设计实验并分析数据。结果表明,培养基中的葡萄糖、酵母浸膏、培养时间是影响甘露醇产量的主要因素。优化后的培养基组成(g/L)为:葡萄糖45.5,酵母浸膏2.2,蛋白胨2.0,pH7.0,28℃静置培养17d。在此条件下,甘露醇含量达到最大值6.21%,较优化前的4.82%提高了28.8%。

Plackett-Burman design and response surface methodology were applied to optimize fermentation medium of mannitol. Experimental design and data analysis were obtained by SAS. The results indicated that mannitol yield was mainly influenced by glucose, yeast extract and culture time. The obtained optimal fermentation medium components（g/L） were glucose 45.5, yeast extract 2.2, peptone 2.0, pH 7.0, and static culture 17 days under the temperature of 28 ℃. Under these conditions, the mannitol content could reach 6.21%, which was 28.8% higher than before.