摘要
探讨紫苏甲醇提取物（PLME）的自由基清除能力和对过氧化氢诱发人类角质形成细胞（HaCaT）细胞氧化损伤的保护作用。细胞经氧化损伤造模后,换用含不同浓度（50~200μg/mL）的PLME继续培养24h,MTT法检测细胞生存率,硫代巴比妥酸比色法测定MDA含量。DPPH和羟基自由基清除实验测定PLME的体外自由基清除能力。PLME干预氧化损伤细胞后,细胞生存率上升,且MDA生成量减少,与未干预组相比较差异有统计学意义（P〈0.01）,并显示出剂量效应关系。此外,PLME对DPPH和羟基自由基有强清除能力,其对DPPH自由基半清除剂量IC50=9.07μg/mL,对羟基为IC50=49.28μg/mL。结果提示,PLME具有较强的自由基清除能力,同时还能有效地减少氧化损伤下HaCaT细胞内MDA生成,提高细胞生存率,对细胞应激损伤有保护作用。
To investigated the free radical scavenging activity and protective effect of methanolic extract from Perilla frutescens（PLME） on H2O2-induced oxidative damage in HaCaT heratinocyte. Various concentration （50~200 μg/mL） of PLME were incubated with HaCaT cells pre-treated by H2O2, and the cells were cultivated continually for 24 h. The cell viability was determined by MTT assay, and contents of MDA were determined by thiobarbituric acid reactive substances （TBARS） assay. The cell viability of HaCaT cells was increased and the contents of MDA were decreased after treated by PLME. There was statistical significant difference between the sample treated groups and untreated control groups （P 0.01）. Those results suggested that PLME has strong free radical scavenging activity and protective effect against H2O2-induced keratinocytes oxidative stress.
出处
《食品科技》
CAS
北大核心
2011年第5期187-190,共4页
Food Science and Technology
