表面活性剂-微波法提取野生刺五加黄酮工艺

The extraction of flavones from wild acanthopanax senticosus harms assisted by surfactant-microwave method

利用表面活性剂增溶作用结合微波辐射提取刺五加果实中的总黄酮。提取工艺用用响应面法(RSM)进行优化。在单因素实验基础上选取实验因素水平,根据Box-Behnken中心组合实验设计原理采用3因素3水平的响应面分析法。在分析了各个因素的显著性和交互作用后,得出刺五加果中总黄酮的最佳提取工艺条件为:微波提取温度60℃、微波提取时间4min、椰子油脂肪酸二乙醇胺0.76%、液料比49:1(mL:g)。在该条件下,刺五加果实中总黄酮的提取率为8.51%。

Surfactant and microwave extraction was applied to gain flavonoids from the acanthopanax senticosus harms. Response surface methodology （RSM） was used to optimize the extraction conditions. Based on the signal factor test, according a three-factor and three-level Box-Behnken central composite design principles, the method of RSM was adopted. Then the factors influencing the technological parameters were determined by means of regression analysis. After analysis on each significant and interaction effect, the results showed that the optimal microwave extraction assisted with surfactant conditions as follows： microwave extraction temperature 60 ℃, extraction time 4 min, the concentration of diethanolamine of coconut oil fatty alcohol 0.76%, the ratio of liquid to material of 49：1（mL：g）, under such condition that the extraction rate of acanthopanax senticosus harms flavonoid could reached 8.51%.