牦牛乳腺上皮细胞-小鼠骨髓瘤细胞化学融合方法的研究

The study of methods on chemicalfusion between yak mammary epithelial cells and murine myeloma cells

采用化学诱导的方法,选择不同分子量的PEG作为诱导剂,成功实现了牦牛乳腺上皮细胞与小鼠骨髓瘤细胞SP2/0的融合,确定了PEG分子量为6000,浓度为50%,作用时间为90s的最佳融合条件.

Yak mammary epithelial cells are successfully fused with murine myeloma cells（SP2/0） after comparing polyehtylene glycol（PEG） inducers with different molecular weights.It is found that the optimized fusion conditions are PEG with 6000 molecular weight and 50% concentration for 90 s incubation.