摘要
目的:观察异氟烷麻醉对大鼠学习记忆的影响。方法:选择学习记忆功能正常的雄性SD大鼠32只,将其随机分为对照组(A组)和异氟烷组(B组),每组16只。两组分别于水合氯醛腹腔注射麻醉后,A组不做处理;B组1%~1.5%异氟烷吸入麻醉4 h,血压维持在正常生理范围。术前、术后即刻,麻醉后第1、3、7天利用Y迷宫进行认知功能的测定和海马组织HE染色观察形态学变化和用高倍镜计数S-100β免疫反应阳性细胞。结果:麻醉前大鼠生理指标和学习记忆功能组间比较差异无统计学意义。①麻醉后第1天正确反应次数B组<A组,全天总反应时间B组>A组,但差异无统计学意义(P>0.05);麻醉后第3、7天两组间正确反应次数和全天总反应时间比较差异无统计学意义(P>0.05)。②麻醉后即刻、麻醉后1 d,B组大鼠海马CAI区S-100β阳性细胞增多,密度略高于A组,差异无统计学意义(P>0.05);B组麻醉后第3、7天海马各区S-100β阳性细胞密度降低,两组间比较差异无统计学意义(P>0.05)。结论:异氟烷麻醉可能轻度影响大鼠脑海马S-100β阳性细胞,但对认知功能没有明显影响。
Objective:To observe the effect of Isoflurane anesthesia on cognitive function in rat.Methods:The author selected 32 male SD rats with normal learning and memory function,which were randomly divided into two groups:control group(group A,n=16) and Isoflurane group(group B,n=16).Anesthesia induction with Chloral Hydrate injected peritoneally in two groups.In group A,rats were without CH treatment;In group B,rats were under 1%-1.5% Isoflurane inhalation to maintain blood pressure at normal physiological range.The anesthesia time in group A was 4 hours,before and after anesthesia,the cognitive function was determined by Y maze at 1st,3rd,7th day postanesthesia,and positive S-100β immunoreactive cells in hippocampus were stained and counted the number by HE.Results:There was no significant difference between groups about physiological index and learning and memory function before anesthesia.①The group B smaller than the group A in the right number and total reaction time at 1st day after anesthesia,the difference was no significant(P0.05);there was no significant difference in the right number and total reaction time at 3rd day or 7th day after anesthesia(P0.05).② Hippocampus S-100β positive cells at right after anesthesia and at 1st day after anesthesia were slightly increased in group B,higher than that of in group A,the difference was no significant(P0.05);hippocampus S-100β positive cells at 3rd day or 7th day after anesthesia had no significant differences between group A and group B(P0.05).Conclusion:Isoflurane anesthesia may slightly affect positive S-100β immunoreactive cells in hippocampus,whereas can not affect cognition function in rat.
出处
《中国医药导报》
CAS
2011年第15期52-54,共3页
China Medical Herald
基金
2008年辽宁省教育厅基金(项目名称:吸入麻醉药与冠心病病人氨基酸代谢及对血管内皮细胞NADPH氧化酶的影响机制
项目编号:2008834)
关键词
异氟烷
麻醉
术后认知功能
Y-电迷宫
S-100Β
Isoflurane
Anesthesia
Postoperation cognitive impairment
Y-type electric maze
S-100β