异氟烷麻醉对大鼠认知功能的影响

The effect on cognitive function of rats during Isoflurane anesthesia

目的:观察异氟烷麻醉对大鼠学习记忆的影响。方法:选择学习记忆功能正常的雄性SD大鼠32只,将其随机分为对照组(A组)和异氟烷组(B组),每组16只。两组分别于水合氯醛腹腔注射麻醉后,A组不做处理;B组1%～1.5%异氟烷吸入麻醉4 h,血压维持在正常生理范围。术前、术后即刻,麻醉后第1、3、7天利用Y迷宫进行认知功能的测定和海马组织HE染色观察形态学变化和用高倍镜计数S-100β免疫反应阳性细胞。结果:麻醉前大鼠生理指标和学习记忆功能组间比较差异无统计学意义。①麻醉后第1天正确反应次数B组<A组,全天总反应时间B组>A组,但差异无统计学意义(P>0.05);麻醉后第3、7天两组间正确反应次数和全天总反应时间比较差异无统计学意义(P>0.05)。②麻醉后即刻、麻醉后1 d,B组大鼠海马CAI区S-100β阳性细胞增多,密度略高于A组,差异无统计学意义(P>0.05);B组麻醉后第3、7天海马各区S-100β阳性细胞密度降低,两组间比较差异无统计学意义(P>0.05)。结论:异氟烷麻醉可能轻度影响大鼠脑海马S-100β阳性细胞,但对认知功能没有明显影响。

Objective：To observe the effect of Isoflurane anesthesia on cognitive function in rat.Methods：The author selected 32 male SD rats with normal learning and memory function,which were randomly divided into two groups：control group（group A,n=16） and Isoflurane group（group B,n=16）.Anesthesia induction with Chloral Hydrate injected peritoneally in two groups.In group A,rats were without CH treatment;In group B,rats were under 1%-1.5% Isoflurane inhalation to maintain blood pressure at normal physiological range.The anesthesia time in group A was 4 hours,before and after anesthesia,the cognitive function was determined by Y maze at 1st,3rd,7th day postanesthesia,and positive S-100β immunoreactive cells in hippocampus were stained and counted the number by HE.Results：There was no significant difference between groups about physiological index and learning and memory function before anesthesia.①The group B smaller than the group A in the right number and total reaction time at 1st day after anesthesia,the difference was no significant（P0.05）;there was no significant difference in the right number and total reaction time at 3rd day or 7th day after anesthesia（P0.05）.② Hippocampus S-100β positive cells at right after anesthesia and at 1st day after anesthesia were slightly increased in group B,higher than that of in group A,the difference was no significant（P0.05）;hippocampus S-100β positive cells at 3rd day or 7th day after anesthesia had no significant differences between group A and group B（P0.05）.Conclusion：Isoflurane anesthesia may slightly affect positive S-100β immunoreactive cells in hippocampus,whereas can not affect cognition function in rat.