高效液相色谱法测定蒲地蓝消炎片中咖啡酸、绿原酸的含量

High performance liquid chromatography in determination on contents of caffeic acid and chlorogenic acid in Pudilan Xiaoyan Tablets

目的:采用高效液相色谱法测定蒲地蓝消炎片中咖啡酸、绿原酸的含量。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-乙腈-0.05%磷酸溶液为流动相(5∶5∶90),检测波长:328 nm,流速:0.8 ml/min,进样量:10μl,柱温:35℃。结果:咖啡酸、绿原酸的线性范围分别为2.162～25.944μg/ml(r=1.000 0,n=7)、0.607 2～7.2864μg/ml(r=1.000 0,n=7),平均回收率分别为98.29%(RSD为0.48%)、98.36%(RSD为0.81%)。结论:所建立的方法快速、简便、准确,可用于蒲地蓝消炎片的质量控制。

Objective：To develop the High performance liquid chromatography（HPLC） for the determination on contents of caffeic acid and chlorogenic acid in Pudilan Xiaoyan Tablets.Methods：The separation was performed on an Agilent ZORBAX SB-C18 column（4.6 mm×250 mm,5 μm） with methanol-acetonitrile-0.05% phosphoric acid（5∶5∶90） as mobile phase.The detective UV wavelength was at 328 nm,the flow rate was 0.8 ml/min,the column temperature was 35℃.Results：The quantitative line range of caffeic acid and chlorogenic acid were 2.162-25.944 μg/ml（r=1.000 0,n=7） and 0.607 2-7.286 4 μg/ml（r=1.000 0,n=7） respectively;the average recoveries of caffeic acid and chlorogenic acid were 98.29%（RSD=0.48%）,98.36%（RSD=0.81%）.Conclusion：The method is rapid,simple and accurate,and can be applied for the quality control of Pudilan Xiaoyan Tablets.