肿瘤坏死因子-α-863位点基因多态性与痛风相关性的初步研究

Association study between polymorphism of tumour necrosis factor-or-863 and gout

目的探讨肿瘤坏死因子-α（TNF—α）-863位点基因多态性与唐山地区汉族人群痛风的相关性。方法以聚合酶链反应-连接酶检测反应（PCR—LDR）方法对80例痛风患者（病例组）和80例正常对照者（对照组）进行TNF-α-863位点的单核苷酸多态性分型，比较各基因型及等位基因频率在正常人群和痛风患者中的差异。结果2组之间TNF—α-863位点各基因型分布频率，病例组与对照组A／A基因型频率相同（均为3．8％），病例组与对照组C／A基因型频率分别为23．8％与38．8％，C／C基因型频率分别为72．5％与57．5％，2组各基因型频率差异无统计学意义（x^2=4．2646，P=0．1187）；病例组与对照组c等位基因频率分别为84．4％与76．9％，差异无统计学意义（x^2=2．8807，P=0．0897）。结论TNF—α-863位点基因多态性可能与唐山汉族人群痛风易感性无关。

Objective To investigate the association between the tumour necrosis factor-a-863 （TNF-α- 863） polymorphism and gout in Han population from the city of Tangshan. Methods We recruited 80 gout patients and 80 healthy individuals into this study. The polymorphisms of TNF-α-863 site were analyzed by polymerase chain reaction-ligase detection reaction （PCR-LDR）. The frequencies of different TNF-α-863 genotypes/alleles were analyzed in the gout group and the control subjects. Results No significant differences were observed in the genotype frequencies （X = 2. 8807, P = 0. 0897 ） and allelic frequencies（ X = 4. 2646, P = 0. 1187） of TNF-α-863 site in the comparison between gout and control groups. Conclusion The result of our study suggests that the polymorphism of TNF-α-863 site may not related to gout in Han population in Tangshan.