离子印迹聚合物在线分离富集-流动注射-化学发光法测定铜

Flow-Injection Chemiluminescence Determination of Trace Copper On-Line Separation and Enrichment by Ion Imprinted Polymer

研究了以铜（Ⅱ）离子作为模板离子,合成了铜（Ⅱ）离子印迹聚合物并将其做成固相萃取柱,直接安装在流动注射系统上,对样品中的铜（Ⅱ）离子进行在线分离富集;经H2SO4＋乙醇混合溶液洗脱液在线洗脱后。在H2SO4介质中,铜（Ⅱ）离子催化高锰酸钾氧化桑色素发生化学发光反应。据此建立了灵敏简单流动注射化学发光法测定铜的新方法。在最佳实验条件下,测定铜的线性范围为0.1~10μg.mL-1,相关系数r=0.996 8,方法检出限为0.001 2μg.mL-1,对4.0μg.mL-1铜进行9次测定的相对标准偏差小于3%,加标回收率在95%~105%之间,方法用于样品中铜的测定,结果满意。

In the present study,Cu（Ⅱ）-imprinted polymer was prepared and used as an effective method for separation and enrichment of Cu（Ⅱ） ion in samples.An on-line preconcentration and extraction flow-injection system using solid phase extraction（SPE） column packed with prepared ion imprinted polymer（IIP） was set up.The column was eluted with a mixture of ethanol and H2SO4 after preconcentration.A sensitive and simple chemiluminescence（CL） method using flow-injection has been developed for the determination of copper.The method is based on the fact that traces of Cu（Ⅱ） exert a catalytic action on the oxidation between morin by KMnO4 solution.After optimizing the different experimental parameters,a calibration graph was obtained over the concentration range of 0 to 10 μg·mL-1 for Cu（Ⅱ） and r=0.996 8.The detection limit is 0.001 2 μg·mL-1.The recoveries are between 95% and 105% and the standard relative deviations（RSD） are less than 3% at 4.0 μg·mL-1（n=9） level was obtained.The new chemiluminescence determination for copper has been established.The method can be satisfactorily applied to the determination of copper in sample.