PNGF复合材料对PC12细胞GAP43和β-TubulinⅡ基因的调控

PNGF Composites Regulated GAP43 and β-TubulinⅡ Genes Transcription in Rat Pheochromocytoma Cells

应用自行设计的RGD接枝的聚(羟基乙酸-L-赖氨酸-乳酸),复合聚乳酸、b-磷酸三钙和神经生长因子(NGF)制备神经导管材料(PNGF)。在体外制备材料浸提液,与PC12细胞共培养1 d、2 d和3 d,分别提取总RNA,通过实时定量PCR试验方法,观察PC12细胞GAP43和β-TubulinⅡ基因表达的变化。结果显示GAP43和β-TubulinⅡ基因的表达量明显高于对照组,其峰值出现在第二天。材料浸提液中NGF在诱导PC12细胞分化中起到显著作用。

A novel nerve guidance conduit（NGC） was synthesized by embedding nerve growth factor（NGF） into a degradable RGD-modified composite（PRGD） to improve the peripheral nerve repair（PNGF）.This study was to evaluate the responses of GAP43 and β-Tubulin Ⅱgenes mRNA expression when PC12 cells were exposed to the extract of PNGF films.RNA was isolated from PC12 cells cultured in vitro and gene expression were detected by real-time quantitative PCR at 1 d,2 d and 3 d.Results indicated that the transcription of GAP43 and β-Tubulin Ⅱgenes were significantly up-regulation in the extract of PNGF films and the peak were almost presented at the second day.Results show that the sustained released NGF promoted PC12 cells differentiation.