高效木质素降解菌株的分离筛选

Isolation and screening of fungal strains with high ligninolytic enzyme activities

为筛选能高效降解木质素的菌株,从31份采集的朽木和土壤样品中分离纯化获得155株菌;将纯化获得的菌株接入到含愈创木酚的选择培养基上进行初筛,观察其颜色变化,根据菌落圈和变色圈直径的比值,筛选出9株可降解木质素的菌株;将这9株菌接入产酶培养基中进行液体静止培养并测定木质素酶活力,最终获得2株高产木质素酶的真菌菌株14-7和15-1.将这2株真菌培养7 d后,所产木质素过氧化物酶、锰过氧化物酶和漆酶的活力分别为0.087、0.060、0.144和0.070、0.059、0.000U·mL-1.

The 155 strains were isolated from rot-wood and soil to screen the strains with high ligninolytic enzyme activities.Nine fungi which showed color-zone in the guaiacols medium were obtained.By measuring the enzyme activity of static liquid culture filtrates,two fungal strains（termed 14-7 and 15-1,respectively） with higher ligninase activity of those were obtained.The activities of lignin peroxidase,manganese peroxidase and laccase from fungal strain 14-7 were up to 0.087,0.060,and 0.144 U·mL^-1,respectively,after 7 d fermentation,and the ones from strain 15-1 were up to 0.070,0.059,and 0.000 U·mL^-1,respectively.