均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺

Optimal process for semi-bionic extraction of bioactive compounds against gastric cancer from water chestnut shell by uniform designation

以SGC7901胃癌细胞存活率为指标,运用半仿生提取方法回收菱壳中的抗胃癌活性成分,考察提取时间、料液比、胃蛋白酶用量、胰蛋白酶用量对提取物抗胃癌活性的影响,并运用均匀设计法确定其最佳提取工艺.结果表明:菱壳与pH≈2提取液按料液质量体积比1∶10,不加入胃蛋白酶,37℃提取30 min后,离心收集沉淀,添加4%胰蛋白酶,菱壳与pH≈8提取液按料液质量体积比1∶10,37℃提取30 min,可获得最佳抑制效果,细胞存活率仅为45.52%.本实验表明,采用半仿生法提取菱壳中的抗胃癌活性成分是可行的.

The viability rate of SGC7901 gastric cancer cells was used as an index,the anti-gastric cancer ingredients from the water chestnut shell were reclaimed by semi-bionic extraction.The effects of extraction time,ratio of water to water chestnut shell,amount of pepsin and trypsin on extraction anti-gastric cancer activity were investigated,and the uniform designation was employed to determine the optimum extraction conditions.The results showed that when 1 g shell powder was added to 10 mL solvent（1∶10 ratio of powder to pH≈2 solvent） in the absence of pepsin,extracting for 30 min at 37 ℃,and then being centrifuged to collect precipitate,adding 10 mL pH≈8 solvent and 4% trypsin,extracting for 30 min at 37 ℃,the extracts had the highest activity against gastric cancer and the cell viability was the lowest,i.e.45.52%.Also,this experiment confirmed that water chestnut shell extracts still had a high activity of inhibiting the growth of gastric cancer cells through semi-bionic extraction.