用乙肝疫苗免疫前后猪淋巴细胞增殖的动态观察

OBSERVATION ON DYNAMICS OF PORCINE LYMPHOCYTE PROLIFERATION IMMUNIAED WITH HBV—VACCINE

用~3H—TdR掺入DNA法体外检测了5只猪用纯化的人工肝疫苗免疫前后的淋巴细胞增殖水平。结果免疫后20d,PHA诱导的淋巴细胞增殖的cpm值显著升高(P<0.01)。免疫后40d,加入乙肝疫苗培养的实验组的cpm值比空白对照组明显升高(P<0.05);加入乙肝疫苗和HBV—TF的PHA诱导试验组均比单独PHA诱导的cpm值升高。

The comparisions of lymphocyte proliferation of five health pigs being immuniaed with purified human HBV—vaccine after and before was investigated in our study, using the assay of ~3H—TdR uptake into DNA of blood. It was found that; 1. Being immuniaed for twenty days, the PHA induced porcine peripheral blood Lymphocyte proliferation very significantly different from that before immunization(P<0.01). 2. being immunized for forty days the lymphocyte proliferation of HBV—vaccine added group was significantly different from blank control(P<0.05); both groups of HBV—vaccine and HBV—TF induced with PHA were significantly different from the control group with PHA added only(P<0.01, P<0.05).