风湿热患者血清乳酸脱氢酶及其同工酶谱

LACTATE DEHYDROGENASE AND IT'S ISOENZYMES PATTERNS IN SERUM OF PATIENT WITH RHEUMATIC DISEASE

对30例风湿热患者血清乳酸脱氢酶(LDH)及其同工酶(LDHi)进行了测定,LDH总活性为500.5±281.6u/dl((?)±s),>550u/dl者占26.7％(8/30);LDH_1谱以LDH_3含量增高为主,LDH_1含量降低,出现LDHi谱以LDH_3含量倒置现象,LDH_3/LDH_1,比值为1.18±0.42((?)±s),与正常对照组(0.73±0.11)相比差异有非常显著性(P<0.001),此比值>0.94(正常对照组上限)为异常指标,其诊断特异性为66.7％。LDH_3/LDH_1比值的大小与LDH总活性及抗“O”的高低无显著性意义(x^2=0.11,P>0.05)。结果表明,测定血清LDH及LDH_1对风湿热的诊断有一定临床意义。

Concentration of tatal lactate dehydrogenase(LDH; EC1. 1. 1.27) and LDH isoenayme patterns were studied in serum of 30 patients with Rheumatic disease and in 29 healthy controls. The LDH values were found to be significantly higher(P<0.001) in patients than in controls, the (?)±s values being 500.5±231.6 and 303.8±104.0u/dl, respectively. Whereas 26.7％ of patients, the LDH values controls, the patients had slight diminution in LDH_1, but increased LDH_3. LDH_3/LDH_1 ratios were found to be significantly higher(P<0.001) in patients than in healthy controls, the (?)±s values being 1.18±0.42 and 0.73±0.11, respectively. In 20 of these patiens(66.7％), the LDH_3/LDH_1 ratios had abovenormal, >0.94. We conclude that determination of LDH and its diagnosing Rheumatic disease.