摘要
该文以产气气杆菌为出发菌株,经紫外线和甲基磺酸乙酯(EMS)复合诱变,从大量突变株中筛选出一株产酶稳定且酶活较高的菌株UV2-10-6,酶活由最初的2.9592U/mL提高为14.82U/mL,提高了4倍。并探讨了一种高效的异淀粉酶产生菌的选育方法,为下一步培养基优化打下基础。
Using Aerobacter aerogenes as the original strain, mutagenesis was taken by application of complex of UV and EMS. The mutant strain UV2-10-6 had high and stable enzyme production was obtained from many mutants. The enzyme activity increased from 2. 9592U/ml to 14. 82U/ml, which was 4 times as from original strain. A new efficient starch-hydrolysis enzyme screening method was investigated, which made a good foundation for medium optimization.
出处
《中国酿造》
CAS
北大核心
2011年第5期45-48,共4页
China Brewing
基金
国家自然科学基金项目资助(31000823)
山东省自然科学基金项目资助(ZR2010CM050)
关键词
异淀粉酶
产气气杆菌
筛选方法
诱变处理
isoamylase
Aerobacter aerogenes
screening method
mutant treatment
