Emdogain和辛伐他汀联合应用对人牙周膜细胞碱性磷酸酶活性的影响

Effects of Emdogain and Simvastatin on the ALP Activity in Human Periodontal Ligament Cells

目的观察Emdogain、辛伐他汀联合应用对人牙周膜细胞碱性磷酸酶活性的影响。方法取培养至第五代的人牙周膜细胞,分别用含50μg/mL Emdogain、含0.125μmol/L辛伐他汀、同时含50μg/mL Emdogain和0.125μmol/L辛伐他汀以及不含上述药物的4组无血清DMEM培养液培养,3 d后酶标仪检测各组细胞的碱性磷酸酶活性。结果仅含Emdogain、仅含辛伐他汀以及不含上述药物的培养液培养的人牙周膜细胞碱性磷酸酶光密度值分别为0.193±0.002、0.197±0.003及0.166±0.002。同时含Emdogain和辛伐他汀的培养液培养的细胞碱性磷酸酶光密度值为0.325±0.002,与其他3组比较差异均有统计学意义(P<0.05)。结论 Emdogain和辛伐他汀联合应用对牙周膜细胞碱性磷酸酶活性有促进作用,提示可能有利于牙周组织的再生。

Objective To observe the effects of combinated Emdogain and Simvastatin on the activity of ALP in hu-man periodontal ligament cells（HPLCs）.Methods Four groups were enrolled including control group 1（to cultureP5HPLCs in DMEM with 50μg/mL Emdogain for 3 days）,control group 2（to culture P5HPLCs in DMEM with0.125 μmol/L Simvastatins for 3 days）,control group 3（to culture P5HPLCs in DMEM without Simvastatins and Emdogain for 3 days）,and treated group（to culture P5HPLCs in DMEM with 0.125 μmol/L Simvastatins and 50μg/mL Emdogain for 3 days）.Then the activity of ALP activity in P5HPLCs was observed by PNPP.Results Optical density of ALP in control group 1 is 0.193±0.002;optical density of ALP in control group 2 is 0.197±0.003;optical density of ALP in control group 3 is 0.166±0.002;optical density of ALP in treated group is 0.325±0.002.Compared with control groups,treated group can increase the ALP activity of HPLCs（P0.05）.Conclusion The combination of Emdogain and Simvastatin showed positive effects on the ALP activity of HPLCs,and it may give experimental proofs to the treatment of periodontal disease associated with cardiovascular disease and diabetes.