摘要
建立咳喘宁中罂粟壳和麻黄所含5种主要生物碱类成分的含量测定方法。样品经固相萃取后用超高效液相色谱法测定,色谱柱为Acquity UPLC BEH C18(100 mm×2.1 mm ID,1.7μm),流动相为乙腈与0.1%磷酸溶液梯度洗脱,检测波长为210 nm(吗啡、磷酸可待因、盐酸麻黄碱和盐酸伪麻黄碱)与251 nm(盐酸罂粟碱),流速0.4 mL·min-1,柱温30℃。结果显示,吗啡、磷酸可待因、盐酸罂粟碱、盐酸麻黄碱和盐酸伪麻黄碱分别在0.375 0~12.50、0.064 32~2.144、0.030 06~1.002、1.126~37.52、0.287 8~9.592μg·mL-1内具有良好的线性关系(r=0.999 7),平均回收率分别为(n=9)99.26%、100.6%、95.29%、100.1%和97.48%。该方法快速、准确、重复性好,可为咳喘宁的质量控制提供快速准确的检测。
The paper is to establish a method for simultaneous determination of 5 kinds of alkaloids in ephedra and poppy which are in Kechuanning tablets. Solid-phase extraction (SPE) was adopted in pretreatment, and a UPLC method with 2 different wavelengths had been developed: 210 nm for the detection of morphine, codeine phosphate, ephedrine hydrochloride and pseudoephedrine hydrochloride, and 251 nm for papaverine hydrochloride. The column used was Acquity UPLC BEH C18 (100 mm x 2.1 mm ID, 1.7μm) with linear gradient elution using acetonitrile and 0.1% phosphoric acid. The flow rate was 0.4 mL.min-1, and the column temperature was 30 ~C. The linear response range was 0.375 0 - 12.50 μg·mL^-l for morphine, 0.064 32 - 2.144 μg·mL^-1 for codeine phosphate, 0.030 06 - 1.002 μg·mL^-1 for papaverine hydrochloride, 1.126 - 37.52 μg·mL^-1 for ephedrine hydrochloride, 0.287 8 - 9.592 μg·mL^-1 for pseudoephedrine hydrochloride (r = 0.999 7). The average recoveries of these compounds were 99.26%, 100.6%, 95.29%, 100.1% and 97.48%, respectively. This is a more reasonable and credible method of quality control for Kechuanning tablets.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第5期581-585,共5页
Acta Pharmaceutica Sinica
基金
国家药品标准提高研究项目(10-199)