尼美舒利对不同COX-2表达水平的食管鳞癌细胞的抑制作用

Inhibitory effect of Nimesulide on esophageal squamous cell carcinoma cell lines with different level of Cyclooxygenase-2 expression

目的:比较选择性环氧合酶-2(COX-2)抑制剂尼美舒利对不同COX-2表达水平的食管鳞癌细胞的抑制作用。方法:选取食管鳞癌细胞株EC 109、KYSE 150和TE-1,采用Western blot方法测定COX-2蛋白表达、MTT法检测细胞增殖抑制,流式细胞术检测细胞周期和细胞凋亡,观察尼美舒利对各组细胞的增殖抑制和促凋亡作用。结果:COX-2蛋白在EC 109细胞中呈高表达,KYSE 150细胞中呈中等度表达,TE-1细胞不表达COX-2蛋白。尼美舒利在50μmol/L-400μmol/L浓度区间可抑制EC 109、KYSE 150细胞的增殖(P<0.05),并呈剂量依赖性,在400μmol/L时对TE-1细胞有抑制作用。EC 109细胞尼美舒利的IC50值最低,KYSE150次之,TE-1最高。尼美舒利可使EC 109和KYSE 150的细胞周期阻滞于G0/G1期,并诱导细胞凋亡,但对TE-1细胞无上述作用。结论:尼美舒利对表达COX-2的食管鳞癌细胞有较好的增殖抑制和促凋亡作用。

Objective：To investigate the inhibitory effect of Nimesulide,a selective Cyclooxygenase-2（COX-2） inhibitor,on esophageal squamous cell carcinoma（ESCC） cell lines with or without COX-2 expression.Methods：The human ESCC cell lines EC 109,KYSE 150 and TE-1 were selected in this study.COX-2 expression was detected by Western blot.After 72 hours treatment with Nimesulide,the cell growth inhibition was determined by MTT assay,the cell cycle arrest and apoptosis were detected by flow cytometry.Results：COX-2 protein expression was observed in EC 109 with high level and in KYSE 150 with moderate level,but not in TE-1.Inhibitory effect of Nimesulide was observed in EC 109 and KYSE 150.Nimesulide suppressed proliferation of EC 109 and KYSE 150 from 50μmol/L to 400μmol/L in a dose-dependent manner（P〈0.05）,while only 400μmol/L Nimesulide showed this effect in TE-1.The IC50 of Nimesulide was low in EC 109,moderate in KYSE 150 and high in TE-1.Nimesulide exerted cell cycle arrest in the G0/G1 phase with induction of apoptosis in EC 109 and KYSE 150,but had no such effect in TE-1.Conclusion：The inhibitory effect of Nimesulide on ESCC cell lines may be influenced by the level of COX-2 expression.