端粒酶活性的原位检测在大肠癌早期诊断中的意义

Telomerase activity detected in situ for the early diagnosis of colorectal carcinoma

目的:研究端粒酶活性的原位检测在大肠癌早期诊断中的意义。方法:运用原位杂交技术检测30例大肠癌组织、癌旁组织及其正常黏膜中端粒酶mRNA的表达情况。结果:在30例大肠癌中,端粒酶mRNA的阳性表达率为90.0%,明显高于癌旁组织、正常黏膜组织(P<0.005)。端粒酶活性的表达强度与肿瘤的浸润深度、临床分期以及淋巴结转移显著相关(P<0.05),与性别、年龄以及肿瘤的分化程度无相关性。结论:端粒酶的激活在大肠癌的发生、发展中起到一定的作用,可以将端粒酶作为一种新的肿瘤标记,通过对细胞端粒酶活性的原位检测来协助大肠癌的早期诊断。

Objective： To study the significance of telomerase activity in the early diagnosis of colorectal carcinoma.Methods： Telomerase activity was examined by using In Situ hybridization mRNA of telomerase in cancer tissues,adjacent cancer tissues,normal tissues from patients with colorectal carcinoma.Results： Twenty seven of 30（90.0%） exhibited telomerase activity in cancer tissues compared with 3/30（10.0%） in adjacent cancer tissues,0/30 in normal tissues.There was significant difference between them（P〈0.005）.Telomerase activity level in colorectal carcinoma was significantly associated with the invasion,clinicopathologic stage and lymph node metastasis（P〈0.05）.Conclusion： The expression of telomerase activity plays some role in development of colorectal carcinoma.Telomerase can be a new tumor marker for colorectal carcinoma.There is significance to detect telomerase activity in situ for early diagnosis of colorectal carcinoma.