碳源对卷枝毛霉EIM-10 γ-亚麻酸产量的影响

Influence of Carbon Sources on γ-linolenic Acid Production by Mucor circinelloides EIM-10

目的:研究碳源对卷枝毛霉脂肪酸产量的影响,为代谢调控卷枝毛霉生产γ-亚麻酸奠定基础。方法:测定卷枝毛霉在各种碳源、碳源浓度及碳氮比条件下生物量、油质产量及油脂中GLA含量。结果:卷枝毛霉EIM-10在以葡萄糖为碳源发酵时,油脂得率为2%,油脂中γ-亚麻酸含量为18%;以大豆油为碳源时,其生物量(干重)达到33g/L,油脂占菌丝体干重的35%,GLA的含量为3%。卷枝毛霉EIM-10不能利用醋酸和柠檬酸,可以利用醋酸钠和柠檬酸钠生长但不积累油脂。结论:卷枝毛霉EIM-10脂肪酸从头合成能力不强,能利用外界脂肪酸合成细胞内油脂。

Objective：To study the influence of carbon sources onγ-linolenic acid production by Mucor circinelloides EIM-10 and make a solid foundation for regulating GLA producing of Mucor circinelloides EIM-10.Method： Dry cell weight and lipid yield of mucor circinelloides and GLA of the oil content was determinated.Result：While glucose was used as carbon source,only 2% lipid of its dry cell weight which contained 18%γ-linolenic acid was accumulated by Mucor circinelloides EIM-10;While soybean oil was used as carbon source,the biomass of Mucor circinelloides was 33g/L and the lipid content was 35% of its dry cell weight with a GLA content of 3%.Acetic acid and citric acid could not be utilized but sodium acetate and sodium citrate could be utilized without lipid accumulation by Mucor circinelloides EIM-10.Conclusion：Ectogenesis fatty acid could be utlized to accumulate lipid but little fatty acid could be synthesized to produce oil by the strain Mucor circinelloides EIM-10.