喉鳞状细胞癌组织中骨桥蛋白和p53的表达及其临床意义

Expressions and clinical significances of osteopontin and p53 in laryngeal squamous cell carcinoma tissues

目的研究人喉鳞状细胞癌(LSCC)及其癌旁相对正常组织中骨桥蛋白(OPN)和p53表达水平,并探讨两者的相互关系及其临床病理学意义。方法采用免疫组织化学SP法,对44例LSCC及癌旁组织手术切除标本常规制作石蜡包埋切片,检测OPN和p53的表达,结合喉癌患者的临床病理学特征进行分析。结果①44例喉癌组织中OPN和p53表达阳性率分别为75.0%(33例)和65.9%(29例),癌旁相对正常组织中分别为13.6%(6例)和2.3%(1例);②OPN的表达与颈部淋巴结转移、临床分期、病理组织学分级有关,与性别、年龄、原发部位无相关性;③OPN和p53的表达呈显著正相关(r=0.360,P=0.016)。结论 OPN在喉癌组织中的表达高于癌旁相对正常组织,可能参与了喉癌的发生、发展和转移,有可能成为判断预后的重要生物学标记物。

Objective To study OPN and p53 expressions and detect their correlation and clinicopathological value in laryngeal squamous cell carcinoma （LSCC） and adjacent tissues. Methods The SP immunohistochemical method was used to study the OPN and p53 expressions in routinely embedded sections of surgical specimens of 44 laryngeal squamous cell carcinomas and adjacent tissues, and clinicopathological features were analyzed. Results （1）The positive rates of OPN and p53 protein were 75.0 % （33 cases）and 65.9% （29 cases） in LSCC, and 13.6% （6 cases） and 2.3% （ 1 case） in adjacent tissue respectively; （2）The OPN expression level was significantly correlated with tumor stage, grade and the presence of lymph nodes, but was not correlated with age, gender and primary tumor site; （~）Expression of OPN was positively correlated with that of p53 （ r = 0. 360, P = 0. 016 ）. Conclusions OPN expression is higher in LSCC than in adjacent tissue, and may participate in the development and metastasis of LSCC. OPN might be an important biological marker in LSCC prognosis.