IL-17对鼻息肉中巨噬细胞炎性蛋白-3α的影响

Effects of macrophage inflammatory protein-3α/CCL20 production by interleukin-17 in nasal polyp epithelial cells

目的探讨白介素-17(IL-17)对鼻息肉上皮细胞中巨噬细胞炎性蛋白-3α(macrophage inflammatory protein-3α,MIP-3α/CCL20)的作用。方法离体培养人鼻息肉上皮细胞,不加刺激因子且用IL-17刺激细胞后,用酶联免疫吸附法(ELISA)测定上清液中MIP-3α/CCL20的含量,并用逆转录聚合酶链反应(RT-PCR)检测人鼻息肉上皮细胞中MIP-3α/CCL20mRNA含量的变化。结果 IL-17刺激鼻息肉上皮细胞后,上清液中MIP-3α/CCL20的含量较不加刺激因子时增多,且IL-17对MIP-3α/CCL20的诱导作用具有剂量-时间依从性,同时测定鼻息肉上皮细胞中MIP-3α/CCL20mRNA的相对表达量,结果相似。结论鼻息肉上皮细胞产生MIP-3α/CCL20,而且IL-17对其有诱导作用。MIP-3α/CCL20可能对T细胞和树突状细胞在鼻息肉的聚集中发挥重要的作用。

Objective To explore the effects of macrophage inflammatory protein-3α/CCL20 production by interleu- kin-17 in nasal polyp epithelial cells. Methods Epithelial cell lines,which were established from nasal polyps, were stimulated by culture medium, IL-17. The amount of MIP-3α/CCL20 in the supematants was measured by enzyme-linked immunosorbent assay （ELISA）. MIP-3α/CCL20 mRNA expression was evaluated by reverse transcription-polymerase chain reaction （RT-PCR）. Results Expression levels of MIP-3α/CCL20 in nasal polyps were significantly increased compared with those only in the culture medium. IL-17 induced MIP-3α/CCL20 production by nasal polyp epithelial cells in a dose- and time-dependent manner. Conclusions Nasal polyp epithelial cells, by producing MIP-3α/CCL20, which can be induced by Interleukin-17, may play an important role in the recruitment of T cells and DCs in nasal polyps.