斑秃皮损中CD4、CD8、FasL和穿孔素表达的研究

A Study on Infiltrating T Lymphocytic Phenotype and Detection of Its Roles in the Lesions of Alopecia Areata

目的:通过研究T淋巴细胞杀伤靶细胞的途径和机制,探讨T淋巴细胞在斑秃发病中的作用。方法:采用免疫组化ABC法,检测斑秃和正常组织皮损中CD4、CD8、FasL和穿孔素的表达。结果:在正常组织中CD4、CD8阳性表达,FasL和穿孔素阴性表达。在斑秃皮损中CD4、CD8、FasL均阳性表达,而穿孔素为阴性表达。结论:T淋巴细胞在斑秃发病中起着重要作用,T淋巴细胞可能通过Fas-FasL结合途径引起细胞凋亡,细胞凋亡参与了斑秃的发病。

Objective：To research the role of T lymphocyte in the alopecia areata,we studied the way and transaction of T lymphocyte about how to kill target cells.Methods：Immunohistochemical staining such as ABC method was used to detect CD4、CD8、FasL and perforin in the lesions of alopecia and normal skin.Results：CD4、CD8 were positive expressed and FasL and perforin were negative in lesions of normal skin.All of CD4、CD8 and FasL were positive expressed in lesions of alopecia areata,but perforin was negative.Conclusion：T lymohocytes were very important to alopecia areata.Cytotoxic T lymohocytes might play a role only through Fas-FasL pathway in the apoptosis of alopecia areata.