姜辣素辅助昂丹司琼对顺铂所致大鼠异嗜行为的抑制作用

Ondansetron in combination with gingerol suppresses cisplatin-induced pica in rats

目的:研究姜辣素辅助昂丹司琼对顺铂所致大鼠急性期和延迟期异嗜行为的抑制作用.方法:大鼠随机分成正常对照组、模型组、昂丹司琼组、姜辣素组及姜辣素+昂丹司琼组,记录顺铂注射后0-24h和48-72h各组大鼠摄食量及啃食高岭土量.72h后取延髓极后区及近端空肠组织,免疫组织化学方法测定组织中P物质表达.结果:顺铂注射后0-24h和48-72h内,姜辣素组、昂丹司琼+姜辣素组摄食量较模型组显著增加(t=13.333,15.000,13.8333,15.167,均P<0.01),啃食高岭土量显著减少(t=9.122,12.986,8.525,8.902,均P<0.01);姜辣素组和昂丹司琼+姜辣素组摄食量均显著高于昂丹司琼组(t=8.000,9.667,11.500,12.833,均P<0.01);昂丹司琼+姜辣素组啃食高岭土量显著低于昂丹司琼组(t=2.499,3.999,均P<0.01);姜辣素组啃食高岭土量在0-24h内高于昂丹司琼组(t=1.365,P<0.05),48-72h内低于昂丹司琼组(t=3.622,P<0.01).昂丹司琼+姜辣素组在0-24h内啃食高岭土量显著低于姜辣素组(t=3.865,P<0.01),在48-72h内啃食高岭土量及摄食量与姜辣素组均无显著差异(t=0.377,1.333,均P>0.05).姜辣素组及昂丹司琼+姜辣素组的AP区P物质免疫组织化学表达均少于昂丹司琼组(t=2.217,2.408,均P<0.01).结论:姜辣素具有可促进大鼠摄食并可抑制顺铂所致的异嗜作用,与昂丹司琼联用可有效抑制急性期和延迟期异嗜行为,其机制可能与抑制P物质表达有关.

AIM：To investigate whether ondansetron in combination with gingerol has suppressive effect on cisplatin-induced acute and delayed pica behavior in rats.METHODS：Rats were divided randomly into blank control group,model control group,ondansetron group,gingerol group,and ondan-setron plus gingerol group.Kaolin and food ingestion were recorded during 0-24 h and 48-72 h after cisplatin administration.At 72 h,the area postrema and jejunum tissue samples were taken to detect the immunoexpression of substance P by immunohistocheminstry.RESULTS：During 0-24 h and 48-72 h after cisplatin administration,food ingestion increased significantly（t=13.333,15.000,13.8333,15.167,all P0.01）but kaolin ingestion decreased significantly（t=9.122,12.986,8.525,8.902,all P 0.01）in the gingerol group and ondansetron plus gingerol group compared to the model control group.Compared to the ondansetron group,food ingestion was significantly higher in the gingerol group and ondansetron plus gingerol group（t=8.000,9.667,11.500,12.833,all P0.01）,and kaolin ingestion was significantly lower in the ondansetron plus gingerol group （t=2.499,3.999,all P0.01）.Kaolin ingestion was higher during 0-24 h（t=1.365,P0.05）but lower during 48-72 h（t=3.622,P0.01）in the gingerol group than in the ondansetron group.Compared to the gingerol group,kaolin ingestion decreased significantly in the ondansetron plus gingerol group during 0-24 h（t=3.865,P 0.01）.No significant difference was noted in kaolin and food ingestion during 48-72 h between the ondansetron plus gingerol group and the gingerol group（t=0.377,1.333,all P0.05）.The immunoexpression levels of substance P in the AP of gingerol group and ondansetron plus gingerol group were lower than that in the ondansetron group（t=2.217,2.408,all P0.01）.CONCLUSION：Gingerol is able to improve food ingestion and inhibit cisplatin-induced pica behavior in rats probably by restraining the immunoexpression of substance P.Ondansetron in combination gingerol is able to effectively inhibit cisplatin-induced acute and delayed pica in rats.