肠黏膜细胞因子在肠易激综合征患者回肠及升结肠组织中的表达

Expression of IL-6,IL-23 and TGF-β1 in the mucosa of the ileum and ascending colon of patients with irritable bowel syndrome: an analysis of 110 cases

目的:研究肠易激综合征(IBS)患者回肠末端及升结肠黏膜组织中炎性因子白介素(IL)-6、IL-23及TGF-β1的表达,探讨IBS的发病机制.方法:选取110例IBS患者,另选40例健康体检者为对照,内镜下取回肠及升结肠肠黏膜组织各1块,用免疫组织化学法测定回肠末端及升结肠肠黏膜中IL-6、IL-23及TGF-β1水平.结果:IL-6及IL-23在IBS患者回肠末端及升结肠肠黏膜的表达高于对照组(IL-6:106.45±12.54vs82.45±11.52,t=10.58;107.48±18.75vs91.32±10.45,t=5.16;IL-23:10.68±3.35vs4.78±2.54,t=10.12;11.42±2.87vs5.20±1.85,t=12.76,均P<0.05);TGF-β1在I B S患者回肠末端及升结肠肠黏膜的表达与对照组比较差异无统计学意义(t=1.57,1.69,P>0.05).结论:IBS患者回肠黏膜及升结肠炎性细胞因子表达水平升高,促炎细胞因子与抑炎细胞因子失衡,与IBS内脏感知及排便习惯的改变等症状的发生有一定相关性.

AIM：To investigate the expression of interleukin（IL）-6,IL-23 and transforming growth factor beta1（TGF-β1）in the mucosa of the ileum and ascending colon of patients with irritable bowel syndrome（IBS）.METHODS：One hundred and ten patients with IBS and 40 healthy volunteers were included in the study.Mucosal samples were taken from the ileum and ascending colon of these subjects to detect the expression of IL-6,IL-23 and TGF-β1 by immunohistochemistry.RESULTS：The expression levels of IL-6 and IL-23 in the mucosa of the ileum and ascending colon were higher in IBS patients than in controls（IL-6：106.45±12.54 vs 82.45±11.52,t =10.58;107.48±18.75 vs 91.32±10.45,t=5.16;IL-23：10.68±3.35 vs 4.78±2.54,t=10.12;11.42± 2.87 vs 5.20±1.85,t=12.76,all P0.05）.No significant difference was found in the expression level of TGF-β1 in the mucosa of the ileum and ascending colon between the two groups（t=1.57,1.69,both P0.05）.CONCLUSION：High expression of proinflammatory cytokines in the mucosa of the ileum and ascending colon may have a correlation with visceral perception and altered bowel habits in patients with IBS.