乳酸双歧杆菌喷雾干燥工艺研究

Spray drying technics of Bifidobacterium lactis

为有效保证乳酸双歧杆菌在生产、消费及贮藏过程中的菌体活性,利用喷雾干燥法制备双歧杆菌微胶囊,深入研究了喷雾干燥过程中的工艺参数。通过壁材选择及相关工艺参数的正交分析,结果表明:喷雾干燥过程中的最佳壁材质量浓度为100 g/L,包括β-环糊精与阿拉伯树胶粉,且质量比为4︰6,芯材质量浓度60 g/L,包括海藻糖20 g/L,甘露醇20 g/L,菌泥25 g/L和抗坏血酸0.01 g/L。最佳工艺参数为进风温度(108±2)℃,排风温度(61±3)℃,塔壁温度(50±3)℃及雾化器转速17 000 r/min。在此工艺条件下菌粉的活菌数为3.4×109 g-1,存活率72.12%,包埋率75.88%。

Spray drying of microencapsulation was used to effectively protect the activity of Bifidobacterium lactis during the production and storage,and technics parameters were also tested.Through wall material selection and orthogonal analysis of relevant parameters,the results showed that the best mass concentration of wall-material was 100 g/L,including β-cyclodextrin and gum acacia,and the mass ratio was 4∶6.The mass concentration of core-material was 60 g/L,include trehalose 20 g/L,mannitol 20 g/L,bacteria mire 25 g/L and ascorbic acid 0.01 g/L.The best inlet air temperature was 108±2℃,outlet air temperature was 61±3℃,and tower wall temperature was 50±3℃.Atomizer speed was 17 000 r/min.Under the optimized conditions,the survival bacteria number were over 3.4×109cfu/g,survival rate was 72.12% and encapsulation efficiency was 75.88%.