马鞭草药材的质量标准研究

Quality Control of Verbenae Herba

目的建立马鞭草药材的定性、定量标准。方法采用薄层色谱法鉴别马鞭草药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷成分;以高效液相色谱法测定以上3种成分的含量。色谱柱为Agilent Eclipse XDB C18（4.6 mm×250 mm,5μm）,流动相为乙腈-甲醇-水梯度洗脱,流速为1.0 mL·min-1,柱温为30℃,检测波长为238 nm。结果马鞭草药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷的薄层色谱鉴别特征明显,专属性强;药材中马鞭草苷、戟叶马鞭草苷和毛蕊花苷的线性范围分别为0.05~1.26μg（r=0.999 9）、0.10~2.58μg（r=0.999 8）和0.09~2.20μg（r=0.999 5）,平均回收率（n=9）分别为96.8%（RSD=1.15%）、98.3%（RSD=2.34%）和98.2%（RSD=2.28%）。结论所建立的马鞭草药材定性定量测定方法简单准确,能够为马鞭草药材的质量控制提供有效数据。

OBJECTIVE To establish a method for quality control of Verbenae Herba.METHODS Verbenalin,hastatoside and acteoside in Verbena officinalis L.were identified by TLC.Their contents were determined by HPLC equipped with Agilent Eclipse XDB C18（4.6 mm×250 mm,5 μm） column,the mobile phase consisted of acetonitrile-methanol-water,the anlysis was done at a flow rate of 1.0 mL·min-1,the column temperature was 30 ℃ and the detection wavelength was 238 nm.RESULTS The linearities of verbenalin,hastatoside and acteoside were in the ranges of 0.05-1.26 μg（r=0.999 9）,0.10-2.58 μg（r=0.999 8） and 0.09-2.20 μg（r=0.999 5）,respectively.The average recoveries（n=9） were 96.8%（RSD=1.15%） for verbenalin,98.3%（RSD=2.34%） for hastatoside,and 98.2%（RSD=2.28%） for acteoside.CONCLUSION This method is simple,accurate with good reproducibility.It is suitable for quality control of Verbenae Herba.