HPLC测定注射用亚锡替曲膦(TF药盒)中替曲膦的含量

Determination of Tetrofosmin in Tetrofosmin and Stannous Chloride for Injection (TF Kit) by HPLC

目的建立HPLC测定注射用亚锡替曲膦（TF药盒）中替曲膦含量的方法。方法采用Waters Symmetry C18（4.6mm×150 mm,5μm）的色谱柱,以甲醇-水（85∶15）为流动相,检测波长为215 nm,流速1.0 mL·min-1。结果替曲膦在50~500μg·mL-1内成线性关系,y=1.299×104＋2.495×104x（r=0.999 8）,平均回收率为98.09%,RSD为0.63%。结论本方法操作简便、准确,可用于TF药盒中替曲膦含量的快速定量分析。

OBJECTIVE To establish an HPLC method for determination of tetrofosmin in tetrofosmin and stannous chloride for injection（TF kit）. METHODS HPLC was adopted with Waters Symmetry C18（4.6 mm×150 mm, 5 μm） column as analytical column, methanol-water （85 ∶ 15） as the mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was 215 nm, respectively. RESULTS The linear range of tetrofosmin was 50-500 μg·mL-1, r=0.999 8. The average recovery was 98.09%, RSD was 0.63%. CONCLUSION The method is simple, accurate and suitable for determination of tetrofosmin in TF kit.