葛根提取物抗痛风性关节炎作用及机制初探

Effect of Radix Puerariae Extracts on Acute Gouty Arthritis and Its Mechanisms

目的观察葛根提取物抗痛风性关节炎作用,并初步探讨其作用机制。方法 60只雄性SD大鼠随机分为6组:正常对照组、模型对照组、葛根提取物低(1.75 g.kg-1)、中(3.5 g.kg-1)、高(5.25 g.kg-1)剂量组,秋水仙碱组。造模前灌胃给药3 d,每日1次,正常对照组给予相应体积的水。采用大鼠踝关节穿刺注入微晶尿酸钠(MSU)建立急性痛风性关节炎模型,期间继续给药,统计造模后1～48 h动物关节肿胀度的变化;测定造模后48 h检测动物全血中白细胞、中性粒细胞和淋巴细胞的水平,血清及10%踝关节组织匀浆中NO水平;测定血清尿酸(UA)水平、处死后,解剖、称量、计算脾脏、肾脏和体重的比例。结果葛根提取物能明显抑制痛风性关节炎模型大鼠的踝关节肿胀,降低脾体指数,提升肾体指数;降低炎症组织NO水平,提高血清NO水平;明显降低模型大鼠的白细胞、中性粒细胞和淋巴细胞水平;并能显著降低血清UA水平。结论葛根提取物具有抗痛风性关节炎的作用。能缓解MSU急性痛风性关节炎模型大鼠的关节肿胀情况,缓解由于炎症引起的脾肿大及肾损伤作用,并降低血清UA水平。其抗炎机制可能与加快NO的释放和降低血液中白细胞、中性粒细胞、淋巴细胞水平有关。

Objective To observe the effect of Radix Puerariae extracts（RPE） on acute gouty arthritis model and to explore their therapeutic mechanisms.Methods Sixty SD male rats were randomly divided into 6 groups of normal control group,model control group,low-dose（1.75 g·kg-1） RPE group,middle-dose（3.5 g·kg-1） RPE group and high-dose（5.25 g·kg-1） RPE group,and colchine group.The rats in the medication groups were treated with corresponding medicine once a day for 3 days before modeling,and the normal control group was given the same volume of water.The model of acute gouty arthritis was induced by injection of microcrystal sodium urate（MSU） solution into articular cavity of the ankle joint.The medication was kept on during the modeling.The changes of arthrocele was monitored during 1～ 48 hour（s） of the modeling.The number of white blood cells（WBC）,neutrophil and lymphocytes in the whole blood was observed,nitric oxide（NO） level in the serum and homogenate as well as serum uric acid（UA） was detected,and the weight of spleen and kidney as well as the ratio of them with the body weight was examined 48 hours after the modeling.Results RPE significantly inhibited the articular swelling,decreased the ratio of spleen and body weight,and increased the ratio of kidney and body weight in model mice.Meanwhile,RPE decreased homogenate NO level while increased serum NO level,reduced the number of WBC,neutrophil and lymphocytes as well as serum UA level in model mice.Conclusion RPE exerts certain therapeutic effect on MSU-induced acute gouty arthritis by relieving the joint swelling,enlarged spleen and renal injury caused by the inflammation.The mechanism could be related with the decrease of serum UA,the promotion of NO release and the decrease of WBC,neutrophil and lymphocytes count in blood.