拘束应激诱发小鼠抗流感病毒能力低下与营养型鸡精的改善作用

Anti-influenza Effect of Essence of Chicken in Mice Loaded with Restraint Stress

目的探讨拘束应激诱发小鼠抗流感病毒能力低下与营养型鸡精(Essence of Chicken,EC)的改善作用。方法将雄性昆明小鼠随机分为正常对照组、病毒组、模型组(拘束应激+病毒)、阳性药物(利巴韦林,50 mg.kg-1)组、EC低、高剂量组(12,24 mL.kg-)1。除正常对照组及病毒组外,其余各组在给药的第2天将小鼠拘束负荷18 h,拘束恢复1 d后,除正常组外其他各组小鼠经鼻黏膜感染流感病毒。从感染病毒当天开始连续21d,记录各组小鼠的死亡数目及小鼠的平均存活时间(MDD);计算病毒感染第4天小鼠肺脏指数;观察EC对拘束应激小鼠免疫功能的影响。结果利巴韦林及EC能提高因拘束应激负荷降低的小鼠抗病毒感染能力,延长生存时间和改善肺脏指数。EC能增加拘束小鼠的胸腺指数、脾脏指数和脾淋巴细胞数目,改善脾淋巴细胞亚群Th(CD3+CD4+)(辅助性T淋巴细胞)/Ts(CD3+CD8+)(抑制性T淋巴细胞)的比值,提高脾自然杀伤(NK)细胞活性,从而改善拘束应激小鼠的免疫功能。结论 EC可以通过改善免疫功能来提高拘束应激小鼠的抗病毒能力。

Objective To study the anti-influenza virus effect of Essence of chicken（EC） in mice loaded with restraint stress.Methods Male Kunming mice were randomly divided into normal control group,virus group,model group（virus ＋ restraint stress）,positive group（ribavirin,50 mg·kg-1）,low-dosage EC（12 mL·kg-1） group and high-dosage EC（24 mL·kg-1） group.Except for normal control and virus groups,all mice were given restraint stress for 18 h on the second day of medication.One day after restraint stress,mice were anesthetized by ether and infected intranasally with influenza virus H9N2 diluted in saline.During the experiment,we recorded the survivor rate and mean day to death（MDD） of mice from the day of virus infection to day 21.On day 4 of virus infection,the lung index was examined.In order to further investigate the anti-influenza virus mechanism of EC in stressed mice,we also determined the impact of EC on immune function of restraint stressed mice.Results Ribavirin and EC obviously increased the survivor rate and MDD,decreased lung index in virus infected mice loaded with restraint stress.We further found that EC could improve the immune function in restraint stressed mice through increasing thymus index,spleen index and spleen lymphocyte count,elevating the ratio of lymphocyte subset Th（CD3＋CD4＋） and Ts（CD3＋CD8＋）,and improving the NK cell activity in spleen.Conclusion EC possesses anti-influenza virus effect in mice loaded with restraint stress through improving immune function.