不同程度间歇低氧大鼠血清丙二醛含量、超氧化物歧化酶和谷胱甘肽过氧化物酶活性的变化

Study on malondialdehyde,superoxide dismutase and glutathione peroxidase in serum of rats exposed in different degrees of intermittent hypoxia

目的 通过测定不同程度间歇低氧大鼠血清丙二醛（MDA）含量及部分抗氧化酶的活性,以探讨氧化应激与阻塞性睡眠呼吸暂停模式间歇低氧的关系,为进一步研究阻塞性睡眠呼吸暂停综合征所致心血管并发症的发病机制提供研究基础.方法 160只成年雄性Wistar大鼠随机均分为5组：5%间歇低氧组、7.5%间歇低氧组、10%间歇低氧组、10%持续低氧对照组和常氧对照组.分别于2周、4周、6周、8周测定各组大鼠血清MDA含量、超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GPx）活性.结果 间歇低氧暴露后大鼠血清MDA含量升高,而且间歇低氧程度不同,MDA含量升高的程度不同,5%间歇低氧组MDA含量升高最为明显;间歇低氧暴露后血清SOD和GPx活性降低,暴露6周时变化最为明显.结论 阻塞性睡眠呼吸暂停模式间歇低氧能引起机体发生氧化应激反应,间歇低氧程度不同,所引起的氧化应激反应强度不同,重度间歇低氧引起的氧化应激反应最为明显.

Objective To investigate the relationship of oxidative stress and intermittent hypoxia of obstructive sleep apnea mode and to provide basis for stodying the mechanism of cardiovascular complications induced by obstructive sleep apnea syndrome through detecting the levels of malondialdehyde （MDA）,superoxide dismutase （SOD） and glutathione peroxidase （GPx） in serum of rats exposed in different degrees of intermittent hypoxia. Methods 160 Wistar rats were randomly divided into five groups： 5% intermittent hypoxia group,7. 5% intermittent hypoxia group,10% intermittent hypoxia group,10% continuous hypoxia group,and normoxia group. Eight rats from each group were sacrificed at the 2nd,4th,6th,and 8th week. The levels of MDA,SOD and GPx in serum were measured. Results The content of MDA in the intermittent hypoxia groups increased. The content of MDA varied with different degrees of intermittent hypoxia. The change of MDA in 5% intermittent hypoxia group was the most significant. The activities of SOD and GPx decreased in the intermittent hypoxia groups. The changes of MDA,SOD. and GPx were the most significant at the 6th week of intermittent hypoxia treatment. Conclusions Intermittent hypoxia of obstructive sleep apnea mode can induce oxidative stress. The severity of oxidative stress is strongly correlated with the degree of intermittent hypoxia,and the oxidative stress induced by severe intermittent hypoxia is the most significant.