丹参酮ⅡA磺酸钠对大鼠肢体缺血再灌注所致肺损伤的保护作用

Protective effect of sodium tanshinon Ⅱ A on lung injury induced by limb ischemia-reperfusion in rats

目的 探讨丹参酮ⅡA磺酸钠对大鼠肢体缺血再灌注后肺损伤的影响.方法 实验采用华北煤炭医学院病理生理教研室常规方法复制大鼠肢体缺血再灌注模型,分正常对照组（A组）,缺血再灌注组（B组）和丹参酮组（C组）,观察各组大鼠血浆和肺组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、黄嘌呤氧化酶（XOD）、白介素8（IL-8）和肿瘤坏死因子α（TNF-α）的含量;应用放免法测定血浆血栓素A2代谢产物血栓素B2（TXB2）和前列环素（PGI2）代谢产物6-酮-前列腺素F1α（6-keto-PGF1α）的数值并进行比较;光镜下观察肺组织形态学改变,电镜下观察肺组织超微结构改变.结果 ①光镜下观察：与B组比较,C组肺血管扩张,炎性细胞聚集、附壁,肺组织水肿等情况均有不同程度减轻.电镜下观察：与B组比较,C组线粒体水肿或空泡变性、内膜下基底膜水肿、肺毛细血管内皮细胞肿胀、肺间质水肿等情况明显减轻.②与A组比较,B组血浆、肺组织MDA及XOD均明显升高（P值均＜0.01）,C组均明显低于B组（P值均＜0.01）;与A组比较,B组血浆、肺组织SOD均明显降低（P值均＜0.01）,C组均明显高于B组（P值均＜0.01）.③与A组比较.B组血浆、肺组织IL-8、TNF-α均明显升高（P值均＜0.01）,C组较B组均降低（P＜0.05或P＜0.01）,C组与A组比较差异无统计学意义.④与A组比较,B组TXB2和6-keto-PGF1α均明显升高（P值均＜0.01）,C组均明显低于B组（P值均＜0.01）,C组血清TXB2高于A组（P＜0.05）,C组与A组血清6-keto-PGF1α差异无统计学意义.结论 丹参酮ⅡA磺酸钠可减轻肢体缺血再灌注大鼠肺部的损伤.

Objective To investigate the protective effect of sodium tanshinone Ⅱ A on lung injury induced by limb ischemia-reperfusion in rats. Methods 60 SD rats were randomly divided into three groups：control group （group A,n =20）,limb-ischemia-reperfusion group （group B,n =20）,and tanshinone Ⅱ A group （group C,n =20）. The levels of malondialdehyde （MDA）,superoxide dismutase （SOD）,xanthine oxidase （XOD）,interleukin-8 （IL-8）,and tumor necrosis factor-α （TNF-α） in plasma and lung tissue were measured. The levels of TXB2 and 6-kcto-PGF1α in serum were measured by radioimmunoassay. The morphological and ultrastructure changes of lung tissues were observed under light microscope and electron microscope,respectively. Results ①Under light microscope,compared with group B,expansion of pulmonary vascular,aggregation of inflammatory cell,pulmonary edema relieved in group C. Under electron microscopy,compared with group B,mitochondrial swelling or degeneration,edema of basement membrane,swelling of pulmonary capillary endothelial cell,and pulmonary interstitial edema relieved in group C. ②MDA and XOD in plasma and lung tissue of group B were significantly higher than those in group A （all P 〈0. 01）,and those in group C were significantly lower than those in group B （all P 〈0. 01）. SOD in plasma and lung tissue of group B was significantly lower than that in group A （all P 〈0. 01）. and that in group C was significantly higher than that in group B （all P 〈0. 01）. ③IL-8、TNF-α in serum and lung tissue of group B were significantly higher than those in group A （all P 〈0. 01）,those in group C were lower than those in group B （ P 〈0. 05 or P〈0. 01）,and there was no statistical significance between group C and group A. ④TXB2 and 6-keto-PGF1α in serum of group B were significantly higher than those in group A （all P 〈0. 01）,and those in group C were significantly lower than those in group B （all P 〈0. 01）. TXB2 in serum of group C was higher than that in group A （ P 〈0. 05）. There was no statistical significance on serum 6-keto-PGF1α between group C and group A. Conclusions Tanshinone Ⅱ A can alleviate lung injury of rats with limb ischemia-reperfusion.