巯基乙酸皮肤涂抹对大鼠睾丸和卵巢组织学与超微结构的影响

Affect of TGA on Pathology and Ultrastructure of Testes and Ovaries in Rats by Smearing on Skin

不同剂量的巯基乙酸,经大鼠背部擦皮90天后,引起睾丸和卵巢损伤。睾丸曲细精管排列紊乱,管腔不等,生殖上皮细胞层次减少,精子缺失。精母细胞和精细胞线粒体减少、肿胀、空泡化及崩解,内质网扩张和溶酶体增多。卵巢颗粒细胞空泡化。

In this paper, using solution of TGA ( Thiologlycolic Acid ) in 5%. 0.5%, 0.05% respecti vely by smearing on skin of 45 rats once a week, for 90 days, we observed pathological and ultrastructural ehanges in testes and ovaries.The results showed TGA could cause toxic lesion of testes and ovaries,and there was a cer tain relation of dose-effect between TGA and lesion. The seminiferous tubules of testes arrange disorder, tubelous cavity was unequal, germinal epithelium laminae thinned and apermatids were absent under microscope. By ultrastructurally, there were a decrease in mitochondria and the mitochondria swelling, vaculated and disintegration with endoplasmic reticular expansive and lysosome increased. The granulosa cells of follicle in ovaries vaculated and degradalated were observed as well as.